Related products
Glutamate Antibody (L) – Mouse Monoclonal
€ 449
GABA Antibody Chicken Polyclonal
€ 449
STAINperfect™ immunostaining kit A
€ 299€ 340
Cinnabarinic acid Antibody – Mouse Monoclonal
€ 449
Serotonin Antibody – Mouse Monoclonal
€ 449
Immunohistochemical analysis reveals accumulation of L-Kynurenine within tumor cells of human colorectal cancer (CRC) tissue.Using DiscoveryUltra™ platform (Ventana), a paraffin-embedded tissue section of CRC was subjected to antigen retrieval, and incubation with primary anti-KYN monoclonal antibody. Presence of Kynurenine was then evaluated through the use of a DAB conjugated secondary antibody. Image (x40 magnification) was acquired using Polaris Vectra (Perkin Elmer) automated slide scanner.
L-Kynurenine detection in human brain tissue by IHCDetection of L-Kynurenine in glial cells in human caudate putamen. Paraffin-embedded tissue section was subjected to pH=6 antigen retrieval followed by overnight incubaton with primary antibody (dilution 1/500). After incubation with polymer-conjugated secondary Ab, DAB was used to visualize the staining
L-Kynurenine visualization in human intestinal immune cells by IFImmunofluorescence staining reveals L-Kynurenine accumulation in specific immune cells in human colon tissue. Paraffin-embedded tissue section was subjected to pH=6 antigen retrieval followed by overnight incubation with primary antibody (dilution 1/250). After incubation with Alexa-488 conjugated secondary Ab, epifluorescence microscopy (100X) was used to visualize IF staining.
L-Kynurenine detection in human colon cancer tissue by IHCImmunohistochemical staining of human colorectal cancer tissue shows cytoplasmic accumulation of L-Kynurenine in tumour cells. Paraffin-embedded tissue section was subjected to pH=6 antigen retrieval, and overnight incubation with primary antibody (1/500 dilution). A polymer-conjugated secondary Ab was added and immunostaining was revealed using DAB.
Affinity & Specificity of anti- L-Kynurenine mAb 3D4-F2Competitive ELISA demonstrates that low amounts of L-Kynurenine conjugate are required to abolish antigen-antibody reaction (high affinity), while rising concentrations of 3-OH-Kynurenine conjugate do not affect reaction (high specificity).
L-KynurenineL-Kynurenine, the first stable by-product of the kynurenine pathway, is synthesized from L-Tryptophan by indoleamine 2,3-dioxygenase (IDO1/2) or tryptophan 2,3-dioxygenase (TDO2) enzymes. Acting as an endogenous ligand of Aryl hydrocarbon Receptor (AhR), L-Kunrenine exerts anti-inflammatory effects and promotes glioma progression. L-kynurenine is also widely used as a biomarker of tryptophan catabolism and kynurenine pathway activation in immune-related and neurological disorders.
Key features
| Clonality | Monoclonal antibody (clone 3D4-F2) |
| Host | Mouse |
| Reactivity | Reacts with all species |
| Tested samples | Human & rodent samples (cell cultures, brain, heart, tumor tissues) |
| Staining procedure | Perform heat antigen retrieval (pH=6) before standard IHF/IF staining |
| Format | 50µL (approx. 40 tissue sections) |
Frequently asked questions
L-Kynurenine Antibody – Mouse Monoclonal
Our anti-L-Kynurenine antibody IS1003 enabled to illustrate, for the first time, the presence of L-kynurenine in human brain and tumor samples by IHC and IF. In the literature, the antibody was used to stain for kynurenine in mouse tissues (tumor, brain, heart) and human samples (cell culture, atheroma plaque, and tumor specimens). This mouse monoclonal antibody can be used in combination with other primary antibodies to kynurenine metabolites (rabbit polyclonals available).
€ 449
94 in stock
| Save with quantity discounts | Unit price after discount |
|---|---|
| 3 to 5 antibodies (any reference) | € 399 |
| 6+ antibodies (any reference) | € 379 |
Pay by online or by purchase order
Pay by credit/debit card or generate a quote at checkout (your products will be sent once you provide us with the purchase order)
Fast Worldwide Shipping with Fedex
We ship your order door-to-door in all countries with FedEx International Priority®.
Worry-free Warranty
Made a mistake ordering ? 30-day free return & refund.
Product damaged during shipping ? Free replacement.
Not getting satisfactory results ? If product did not meet quality standards, customer support offers replacement or refund.
Direct Customer Support
For questions, protocol recommendations, and troubleshooting, you have direct access to the scientist that developed the antibody or ELISA kit you’re using.
Direct customer support is available all business days.
A knowledge base containing protocols, Frequently Asked Questions, and troubleshooting tips is also available online.
They published with this product
You may also like…
L-Tryptophan Antibody – Mouse Monoclonal
€ 449
Kynurenine/Kynurenic Acid/Quinolinic Acid ELISA pack (3-KYNs ELISA pack)
€ 1.499€ 1.797
Kynurenine Antibody (L) – Rabbit Polyclonal
€ 449
Quinolinic acid Antibody – Rabbit Polyclonal
€ 449
IS003
L-Kynurenine Antibody – Mouse Monoclonal
Anti-L-Kynurenine mAb – Purified monoclonal antibody for research use only (liquid 50µL)
Specifications
| Product name | L-Kynurenine antibody |
| Synonyms | (S)-Kynurenine antibody
L-2-Amino-4-(2-aminophenyl)-4-oxobutanoic acid antibody Kynurenin antibody 3-Anthraniloyl-L-alanine antibody |
| Immunogen | Conjugated L-Kynurenine |
| Isotype |
IgG1 k chain |
| Clone |
Clone 3D4-F2 |
| Specificity | When tested in competitive ELISA, the anti-L-Kynurenine antibody did not show any significant cross reactivity with L-Tryptophan, 3-hydroxy-DL-Kynurenine, Kynurenic acid, Anthranilic acid or 3-hydroxyAnthranilic acid conjugates. |
| Volume | 50 µL |
| Form | Liquid |
| Purity | Purified IgG |
Instructions
| Storage |
Store at +4°C for short term (6 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Immunohistochemistry (IHC) | Dilute at 1:200-1:2000. Perform heat antigen retrieval (pH=6) before initiating IHC staining protocol on paraffin-embedded and frozen sections |
| Immunofluorescence (IF) |
Dilute at 1:100-1:1000 on paraffin-embedded and frozen sections. Perform heat antigen retrieval and incubate with fluorescent dyes conjugated secondary antibody |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
