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Noradrenaline fibers in the pons of mouse embryo.Noradrenergic fibers were specificaly detected in the medulla of mouse embryo using STAINperfect immunostaining kit A and rabbit polyclonal anti-nordrenaline antibody and following the protocol for whole mount samples. Fluorescent secondary antibodies were used and pictures were acquired by confocal imaging.
Noradrenergic neurons in the pons of embryonic mouse.Using STAINperfect immunostaining kit A, the anti-Noradrenaline rabbit polyclonal antibody (Ref: IS1042) demonstrates the presence of noradrenaline in embryonic mouse pons (E13.5). The staining was performed according to the protocol optimized for whole-mount samples and was revealed using fluorescent conjugated secondary antibody.
Noradrenergic neurons in CNS of mouse embryo.Noradrenergic fibers were specifically detected in the medulla of mouse embryos using the STAINperfect Immunostaining Kit A in combination with a rabbit polyclonal anti-noradrenaline antibody, according to the protocol optimized for whole-mount samples. Fluorescently labeled secondary antibodies were applied, and images were acquired by confocal microscopy.
Norepinephrine fibers in the spinal cord of embryonic mouseNoradrenergic fibers were specifically detected in the medulla of mouse embryos using the STAINperfect Immunostaining Kit A combined with a rabbit polyclonal anti-noradrenaline antibody, following the manufacturer's recommended protocol for whole-mount samples. Fluorescent secondary antibodies were employed, and images were acquired using confocal microscopy.
Affinity & specificity of anti- L-Noradrenaline antibodyCompetitive ELISA demonstrates that low amounts of L-DOPA conjugate are required to abolish antigen-antibody reaction (high affinity), while rising concentrations of L-DOPA competitors Dopamine or Normetanephrine do not affect reaction (high specificity).
Key features
| Clonality | Polyclonal antibody |
| Host | Rabbit |
| Reactivity | Reacts with all species |
| Tested samples | Rodent brain, adipose & spinal cord tissues, and spider brain tissues. |
| Staining procedure | STAINperfect immunostaining kit A (protocols for cell cultures, whole mounts, tissue sections) |
| Format | 50µL (approx. 40 tissue sections) |
Frequently asked questions
L-Noradrenaline Antibody (Norepinephrine) – Rabbit Polyclonal
The anti-Noradrenaline (NA) antibody IS1042 was validated for immunofluorescence in mouse brain and spinal cord tissues, in combination with the STAINperfect immunostaining kit A. This anti-Norepinephrine antibody was recently used to evidence noradrenergic neurons in spider brain (Jin et al, Nature Ecology & Evolution, 2023).
€449
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For questions, protocol recommendations, and troubleshooting, you have direct access to the scientist that developed the antibody or ELISA kit you’re using.
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IS1042
L-Noradrenaline Antibody (Norepinephrine) – Rabbit Polyclonal
Anti-Noradrenaline pAb – Purified polyclonal antibody for research use only (liquid 50µL)
Specifications
| Product name | L-Noradrenaline antibody – Rabbit pAb |
| Synonyms | Anti-Noradrenaline antibody Anti-Norepinephrine antibody Anti-NA antibody |
| Immunogen | Conjugated Noradrenaline |
| Specificity | When tested in competitive ELISA, the anti-conjugated NA antibody did not show any significant cross reactivity with for example Dopamine and normetanephrine conjugates. |
| Volume | 50 µL |
| Form | Liquid |
| Purity | Purified anti-serum |
Instructions
| Storage |
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Immunofluorescence (IF) |
Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample (Cell cultures, Whole mounts, Tissue sections) |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
