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Cross-validation of N8-Acetyl-Spermidine ELISA and LC/MS data in human plasma samplesN8-AcSpdn was quantified in human plasma samples from healthy subjects using IS-I-2500R ELISA kit or by LC/MS. Correlation study showed R2=0.9865, thereby confirming the accuracy of the immunoassay.
Typical standard curve of N8-Acetyl-Spermidine ELISATypical standard curve obtained with the N8-AcSpdn ELISA kit. In this competitive enzyme immunoassay, optical density is inversely correlated with N8-AcSpdn levels within a linear range of 2.4 – 93.75nM. (example data - do not use for calculation).
Key features
| Sample type | Plasma, Cell culture supernatant |
| Capacity | 96-well plate |
| Sensitivity | 0.5nM |
| Range | 2.4 – 93.75nM |
| Assay time | Sample preparation 3h, ELISA overnight |
| Reactivity | Reacts with all species |
| Application | Research use only |
Frequently asked questions
N8-Acetyl-Spermidine ELISA kit
The first and only ELISA kit for the quantification of N8-Acetyl-Spermidine (N8-AcSpdn) in plasma, serum, tissue homogenates and cell supernatants. Highly sensitive, compatible with any species, this kit enables accurate measurements of N8-AcSpdn in low-volume samples (>20µL).
€599
96 in stock
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For questions, protocol recommendations, and troubleshooting, you have direct access to the scientist that developed the antibody or ELISA kit you’re using.
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N1-Acetyl-Spermidine ELISA kit
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IS-I-2500R
N8-Acetyl-Spermidine ELISA kit
N8-Acetyl-Spermidine ELISA kit – Research test for the detection of N8-Acetyl-Spermidine (N8-AcSpdn)
Specifications
| Product name | N8-Acetyl-Spermidine ELISA kit |
| Description | Competitive ELISA kit for the quantitative measurement of N8-Acetyl-Spermidine (N8-AcSpdn) in plasma samples. For research use only |
| Format | 96-well plate |
| Samples | Plasma, Cell culture supernatant |
| Minimal sample volume | 20µL |
| Reactivity | Reacts with all species |
| Standard range | 2.4 – 93.75nM |
| Sensitivity |
0.5nM |
| Specificity | No significant cross-reactivity was observed with N1-Acetyl-Spermidine, Spermidine, Spermine, Ornithine & Putrescine |
| Assay time | Sample preparation 3h and ELISA overnight |
Instructions
| Storage | Store at 2-8°C for to 6 months |
| Sample collection & storage | EDTA Plasma Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months). |
| Sample preparation | Sample preparation (3 hours) |
| ELISA | N8-Acetyl-Spermidine antiserum overnight incubation, revelation and read steps (1h) |
