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Immunofluorescence imaging of Normetanephrine in crayfishNormetanephrine was stained within fibers of crayfish thoracic ganglia. Staining was performed on whole mount nervous chains with Stainperfect immunostaining kit A following whole mount sample protocol. Fluorescent labeled secondary antibody was used and image captured by confocal imaging.
Immunofluorescence imaging of Normetanephrine in crayfishNormetanephrine was detected within mouse adult midbrain in both soma with granular pattern and fibers. Staining was performed after sample preraparation - free floating cryosections - with Stainperfect immunostaining kit A following whole mount protocol. Fluorescent labeled secondary antibody was used and picture obtained by confocal imaging.
Affinity & specificity of anti-Normetanephrine antibodyCompetitive ELISA demonstrates that low amounts of Normetanephrine conjugate are required to abolish antigen-antibody reaction (high affinity), while rising concentrations of Normetanephrine competitors Dopamine or Noradrenaline do not affect reaction (high specificity).
NormetanephrineNormetanephrine was stained within fibers of crayfish thoracic ganglia. Staining was performed on whole mount nervous chains with Stainperfect immunostaining kit A following whole mount sample protocol. Fluorescent labeled secondary antibody was used and image captured by confocal imaging.
Key features
| Clonality | Polyclonal antibody |
| Host | Rabbit |
| Reactivity | Reacts with all species |
| Tested samples | Mouse brain tissues, crayfish tissues |
| Staining procedure | STAINperfect immunostaining kit A (protocols for cell cultures, whole mounts, tissue sections) |
| Format | 50µL (approx. 40 tissue sections) |
Frequently asked questions
Normetanephrine Antibody – Rabbit Polyclonal
The rabbit polyclonal anti-normetanephrine antibody IS1028 was validated for immunohistofluorescence (IF) in crayfish tissues and mouse brain whole mounts using the STAINperfect immunostaining kit A. In combination with this kit, IS1028 can also enable effective visualization of normetanephrine in cell cultures and tissue sections.
€449
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IS1028
Normetanephrine Antibody – Rabbit Polyclonal
Anti-Normetanephrine pAb – Purified polyclonal antibody for research use only (liquid 50µL)
Specifications
| Product name | Normetanephrine antibody |
| Synonyms | Anti-3-O-Methylnoradrenaline antibody |
| Immunogen | Conjugated Normetanephrine |
| Specificity | When tested in competitive ELISA, the anti-conjugated Normetanephrine antibody did not show any significant cross reactivity with Normetanephrine analogs, including metanephrine and noradrenaline. |
| Volume | 50 µL |
| Form | Liquid |
| Purity | Purified anti-serum |
Instructions
| Storage |
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Immunofluorescence (IF) |
Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample (Cell cultures, Whole mounts, Tissue sections) |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
