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Immunolabeling of Octopaminergic and Serotoninergic neuronsImmunostaining of crayfish eyetalk using anti-octopamine rabbit polyclonal antibody (green) and anti-serotonin goat polyclonal antibody (red). Tissus were processed with whole mount protocol of STAINperfect immunostaining kit A. Fluorescent labeled secondary antibodies were used and pictures were acquired by confocal imaging with high magnification.
Fibers of octopaminergic neurons in crayfishOctopaminergic fibers of crayfish brain detected using ImmuSmol rabbit polyclonal anti-Octopamine antibody following whole mount samples protocol of STAINperfect immunostaining kit A. Secondary antibody was used and image captured by confocal microscopy.
Immunofluorescence imaging of Octopamine in crayfish brainAnti-Octopamine antibody highlights the arborization of octopamine neurones in the brain of a crayfish. Staining was performed using STAINperfect immunostaining kit A, following the protocol for whole mount. Fluorescent labeled secondary antibody was used and picture obtained by confocal imaging. This picture highlights the presence of Octopamine within fibers.
Imaging of Octopamine in brain of a crayfishDetection of octopaminergic neurons in the crayfish brain using STAINperfect immunostaining kit A and according to the protocol for whole mount samples. Secondary antibody was used and image obtained by confocal imaging at high magnification. This staining reveals the presence of Octopamine within particular cell bodies.
Affinity & specificity of anti-Octopamine antibodyCompetitive ELISA demonstrates that low amounts of Octopamine conjugate are required to abolish antigen-antibody reaction (high affinity), while rising concentrations of Tyramine and Noradrenaline (Octopamine competitors) do not affect reaction (high specificity).
OctopamineImmunostaining of crayfish eyetalk using anti-octopamine rabbit polyclonal antibody (green) and anti-serotonin goat polyclonal antibody (red). Tissus were processed with whole mount protocol of STAINperfect immunostaining kit A. Fluorescent labeled secondary antibodies were used and pictures were acquired by confocal imaging with high magnification.
Key features
| Clonality | Polyclonal antibody |
| Host | Rabbit |
| Reactivity | Reacts with all species |
| Tested samples | Crayfish tissues |
| Staining procedure | STAINperfect immunostaining kit A (protocols for cell cultures, whole mounts, tissue sections) |
| Format | 50µL (approx. 40 tissue sections) |
Frequently asked questions
Octopamine Antibody – Rabbit Polyclonal
The anti-Octopamine antibody IS1033 was validated for immunofluorescence in crayfish brain tissues, using the STAINperfect immunostaining kit A for sample preparation. In combination with the staining kit, this rabbit polyclonal antibody can label Octopamine in cell cultures and tissues for IF/IHC imaging.
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IS1033
Octopamine Antibody – Rabbit Polyclonal
Anti-Octopamine pAb – Purified polyclonal antibody for research use only (liquid 50µL)
Specifications
| Product name | Octopamine antibody – Rabbit pAb |
| Synonyms | Anti-2-Amino-1-(4-hydroxyphenyl)ethanol polyclonal antibody |
| Immunogen | Conjugated Octopamine |
| Specificity | When tested in competitive ELISA, the anti-conjugated Octopamine antibody did not show any significant cross-reactivity with competitors Tyramine, Dopamine or Noradrenaline conjugates |
| Volume | 50 µL |
| Form | Liquid |
| Purity | Purified anti-serum |
Instructions
| Storage |
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Immunofluorescence (IF) |
Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample (Cell cultures, Whole mounts, Tissue sections) |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
