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Key features
| Sample type | Serum |
| Capacity | 96-well plate |
| Sensitivity | 0.05 ng/mL |
| Range | 0/0.1 – 25.6 ng/mL |
| Assay time | 75 min |
| Reactivity | Human |
| Application | Research use or in vitro diagnostics (IVD in EU only) |
Frequently asked questions
Pregnenolone ELISA kit – Serum Samples
This Pregnenolone ELISA kit is designed for the quantitative measurement of the endogenous steroid pregnenolone in human serum samples. As a crucial downstream product of cholesterol metabolism, pregnenolone is instrumental in confirming the activity of CYP11A1 and 3β-HSD enzymes in both the gonads and adrenal glands. Accurate measurement of pregnenolone can therefore assist in identifying adrenal function disorders, such as congenital adrenal hyperplasia, endocrine tumors, and infertility. Moreover, because pregnenolone is a key precursor in the steroid hormone biosynthesis pathway, this kit is also well-suited for monitoring steroidogenesis—for example, in studies evaluating the impact of endocrine-disrupting chemicals (EDCs).
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Pregnenolone ELISA kit – Serum Samples
Enzyme immunoassay for the direct quantitative determination of pregnenolone in human serum.
Specifications
| Product name | Pregnenolone ELISA |
| Description | In vitro diagnostics / Research enzyme immunoassay (ELISA) for the quantitative determination of pregnenolone in human serum. |
| Format | 96-well plate |
| Samples | Serum |
| Minimal sample volume | 50µL |
| Reactivity | Human |
| Standard range | 0/0.1 – 25.6 ng/mL |
| Sensitivity | 0.05 ng/mL |
| Specificity | No significant cross-reactivity was observed with Pregnenolone analogs |
| Assay time | 75 min |
Instructions
| Storage | Store at 2-8°C for to 6 months |
| Sample collection & storage | Serum: Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months). No specific pre-treatment required. |
| Sample preparation | Enzymatic conjugation (60 min) |
| ELISA | Incubation, revelation and read steps (15 min) |
