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Immunohistochemistry against serotonin (5HT) on a FFPE human colon coronal section.5HT immunoreactivity, revealed using anti-serotonin (5HT, rabbit polyclonal antibody #IS1086), is highlighted in enterochromaffin cells of the intestinal mucosa.
Immunohistochemistry against serotonin (5HT) on a FFPE human colon coronal section5HT immunoreactivity, revealed using anti-serotonin (5HT, rabbit polyclonal antibody #IS1086), is highlighted in enterochromaffin cells of the intestinal mucosa.
Serotonergic neurons of the Raphe nucleus through a FFPE mouse brainstem coronal sectionrevealed by anti-serotonin (5HT, rabbit polyclonal antibody #IS1086) and Microtubule-Associated Protein-2 (MAP2) immunohistofluorescence. 5HT immunoreactivity highlights well the 5HT-containing neurons of the dorsal (DRN).
Affinity & specificity of anti-Serotonin rabbit polyclonal antibodyCompetitive ELISA highlights that low amounts of conjugated Serotonin are required to abolish antigen-antibody reaction (high affinity), while rising concentrations of analog conjugates (5-Hydroxy-Tryptophan, Tryptamine or Tryptophan) affect only to a lesser extent the reaction (high specificity).
Key features
| Clonality | Polyclonal antibody |
| Host | Rabbit |
| Reactivity | Reacts with all species |
| Tested samples | Human tumor samples, Mouse brain tissues |
| Staining procedure | IHC / IHF |
| Format | 50µL (approx. 40 tissue sections) |
Frequently asked questions
Serotonin Antibody for FFPE samples – Rabbit Polyclonal
This novel antibody raised against Serotonin (5-hydroxytryptamine, 5-HT) (#IS1086, polyclonal) is compatible with formalin-fixed biological materials. While allowing for the specific immunodetection of the serotonergic systems, our antibody has been validated for its use through immunochemistry and immunofluorescence applications on formalin-fixed paraffin-embedded (FFPE) samples originating from different species.
€449
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IS1086
Serotonin Antibody for FFPE samples – Rabbit Polyclonal
Anti-Serotonin pAb for FFPE samples – Purified polyclonal antibody for research use only (liquid 50µL)
Specifications
| Product name | Serotonin antibody – 5-HT rabbit polyclonal Ab |
| Synonyms | Anti-5-HT antibody Anti-5-hydroxytryptamine antibody |
| Immunogen | Conjugated 5-HT |
| Specificity | When tested in competitive ELISA, the anti-conjugated serotonin antibody did not cross-react with 5-hydroxytryptamine analogs, including tryptamine and 5-hydroxytryptophan |
| Volume | 50 µL |
| Form | Liquid |
| Purity | Purified anti-serum |
Instructions
| Storage |
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| IHC/IF |
Dilute at 1/250 -1/1000 |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
