Antibodies & immunoassays for small molecules 3-HydroxyAnthranilic acid Monoclonal Antibody
Ref: IS001
IHCKynurenine pathwayMonoclonal AntibodiesICCIF
+IHC validation of anti-3-Hydroxy-Anthranilic acid antibody in human colon tumor
IHC staining shows accumulation of 3-hydroxy-anthranilic acid in human colon cancer cells, as well as in cells from the tumoral microenvironment. Human paraffin-embedded colon tumor tissue was subjected to pH=6 antigen retrieval, and overnight incubation with primary 3-Hydroxy-Anthranilic acid antibody (1/1000 dilution). After polymer-conjugated secondary antibody exposure, staining was observed through DAB coloration.
DatasheetMSDS
The first and only anti-3-HydroxyAnthranilic acid antibody available for research use. The 5B2-G2 primary mouse monoclonal antibody was validated for IHC both in human tumor and brain tissues. When tested by competitive ELISA, the antibody demonstrated strong affinity and high specificity.
| Clonality | Monoclonal antibody (clone 5B2-G2) |
| Host | Mouse |
| Valided applications | IHC |
| Reactivity | Reacts with all species |
| References | Not yet cited to our knowledge Submit content and get a 10% discount! |
| Format | 50µL |
Product overview
| Product name | 3-Hydroxyanthranilic acid antibody |
| Synonyms | Anti-3-Hydroxy-Anthranilic acid antibody
2-Amino-3-hydroxybenzoic acid antibody 3-OH-Anthranilic acid antibody 3-hydroxanthranilate antibody 3-OHAA antibody |
| Immunogen | Conjugated 3-Hydroxyanthranilic acid |
| Isotype | IgG1 k chain |
| Clone | Clone 5B2-G2 |
| Specificity | When tested in competitive ELISA, the anti- 3-HydroxyAnthranilic acid antibody did not show any significant cross reactivity with Anthranilic acid or Cinnabarinic acid conjugates |
Storage
| Form | Liquid |
| Purity | Purified IgG |
| Concentration | 0,5 mg/ml |
| Storage |
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Material safety datasheet | Download MSDS |
| Immunohistochemistry (IHC) | Dilute at 1:200-1:2000. Perform heat antigen retrieval (pH=6) before initiating IHC staining protocol on paraffin-embedded and frozen sections |
| Immunofluorescence (IF) | Dilute at 1:100-1:1000 on paraffin-embedded and frozen sections. Perform heat antigen retrieval and incubate with fluorescent dyes conjugated secondary antibody |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
Product citation
- Indoleamine 2,3-dioxygenase (IDO)-1 and IDO-2 activity and severe course of COVID-19
Check the article
Authors : Guo et al., Journal of Pathology
2022-01
