3-Hydroxyanthranilic acid antibody – Rabbit Polyclonal
Ref: IS1008
Polyclonal AntibodiesICCIFIHCKynurenine pathwayTryptophan metabolism
DatasheetMSDS
This is the first and only anti-3-Hydroxy-Anthranilic acid (3-HAA) rabbit polyclonal antibody available for research use. Confirmed to be highly specific and affine by competitive ELISA, the antibody is currently being validated for IHC and IF use.
Clonality | Polyclonal |
Host | Rabbit |
Valided applications | IHC / IF |
Reactivity | Reacts with all species |
Format | 50µl |
Size | 50µg |
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440.00€
97 in stock
Worldwide shipping
Product overview
Product name | 3-Hydroxyanthranilic acid rabbit polyclonal antibody |
Synonyms | Anti-3-Hydroxy-Anthranilic acid polyclonal antibody
2-Amino-3-hydroxybenzoic acid polyclonal antibody 3-OH-Anthranilic acid polyclonal antibody 3-hydroxanthranilate polyclonal antibody 3-OHAA polyclonal antibody 3-HAA polyclonal antibody |
Immunogen | Conjugated 3-Hydroxyanthranilic acid |
Specificity | When tested by competitive ELISA, the anti- 3-HydroxyAnthranilic acid polyclonal antibody did not show any significant cross reactivity with Anthranilic acid or quinolinic acid conjugates |
Reconstitution & storage
Form | Liquid |
Purity | Purified anti-serum |
Storage | Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
Material safety datasheet | Download MSDS |
Immunocytochemistry(ICC) | Dilute at 1:200-1:2000. Perform heat antigen retrieval (pH=6) before initiating IHC staining protocol on paraffin-embedded and frozen sections |
Immunohistochemistry (IHC) | Dilute at 1:100-1:1000 on paraffin-embedded and frozen sections. Perform heat antigen retrieval and incubate with fluorescent secondary antibody conjugate |
Immunohistofluorescence (IHF) | Dilute at 1:100-1:1000 on paraffin-embedded and frozen sections. Perform heat antigen retrieval and incubate with fluorescent secondary antibody conjugate |
Comments | Optimal working dilutions must be determined by the end-user |
Restrictions | For research use only |
Selected publications on 3-HydroxyAnthranilic acid:
- Lee WS et al. Int Immunopharmacol. The tryptophan metabolite 3-hydroxyanthranilic acid suppresses T cell responses by inhibiting dendritic cell activation. 2013 Nov;17(3):721-6. doi: 10.1016/j.intimp.2013.08.018. Epub 2013 Sep 9.
- Adams S et al. The kynurenine pathway in brain tumor pathogenesis. Cancer Res. 2012 Nov 15;72(22):5649-57. doi: 10.1158/0008-5472.CAN-12-0549. Epub 2012 Nov 9.
- Krause D et al. The tryptophan metabolite 3-hydroxyanthranilic acid plays anti-inflammatory and neuroprotective roles during inflammation: role of hemeoxygenase-1. Am J Pathol. 2011 Sep;179(3):1360-72. doi: 10.1016/j.ajpath.2011.05.048.
- Yan Y et al. IDO upregulates regulatory T cells via tryptophan catabolite and suppresses encephalitogenic T cell responses in experimental autoimmune encephalomyelitis. J Immunol. 2010 Nov 15;185(10):5953-61. doi: 10.4049/jimmunol.1001628. Epub 2010 Oct 13.
- Hayashi T et al. 3-Hydroxyanthranilic acid inhibits PDK1 activation and suppresses experimental asthma by inducing T cell apoptosis. Proc Natl Acad Sci U S A. 2007 Nov 20;104(47):18619-24. Epub 2007 Nov 14.