Antibodies & immunoassays for small molecules Arginine ELISA kit
Ref: IS-I-0400R
Arginine metabolismAmino acidsResearch ELISA kits (RUO)ELISA
+Arginase inhibitor INCB-1158 restores Arg level in sera and TIFs in syngeneic MC38 mouse colorectal cancer model.
C57BL/6J mice were subcutaneously inoculated with MC38 colorectal cancer cell line and treated with 100mg/kg of INCB-1158 for one or five consecutive days. Sera (A) and TIFs (B) were collected after each treatment period and subjected to Arg level quantification using #IS-I-0400R Arg ELISA kit.
DatasheetProtocolMSDS
Arginine (Arg) is a basic non-essential amino acid well known to participate in physiological functions such as i) vascular tone homeostasis as contributing to the production of nitric oxide (NO) and ii) immune response control, through NO synthase (NOS) and Arginase enzymes, respectively.
The Arginine (Arg) ELISA kit allows for the determination of Arg in serum and plasma samples working with a sample volume of 20μL. The kit is easy to implement and well suited for both preclinical and clinical studies.
| Sample type | Serum, Plasma, Cell culture |
| Capacity | 96 tests |
| Sensitivity | 2.1µM |
| Range | 13 – 500µM |
| Assay time | Sample preparation 3h, ELISA overnight |
| Reactivity | Reacts with all species |
Product overview
| Product name | L-Arginine ELISA kit |
| Description | Competitive ELISA kit for the quantitative measurement of Arginine (L-Arg) in serum, plasma and cell culture samples. For research use only |
| Format | 96-well plate |
| Samples | Serum, Plasma, Cell culture |
| Minimal sample volume | 20µL |
| Reactivity | Reacts with all species |
| Standard range | 5.12 – 200µM |
| Sensitivity |
2.1µM |
| Specificity | No significant cross-reactivity was observed with HomoArginine, Agmatine, L-Citruline, L-Ornithine, ADMA and SDMA |
| Assay time | Sample preparation 3h and ELISA overnight |
| Storage | Store at 2-8°C for up to 6 months |
| Datasheets | Instructions for use, Material Safety Datasheet |
| Sample collection & storage | Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands
Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months) |
| Sample preparation | Sample preparation (3 hours) |
| ELISA | L-Arginine antiserum overnight incubation, revelation and read steps (1h) |
| Detailed protocol | Download instructions for use |
Product citation
- Circulating L-Arginine predicts the survival of cancer patients treated with immune checkpoint inhibitors
Check the article
Authors : Peyraud et al., Annals of oncology
2022-07
