Arginine ELISA kit – High Sensitivity – Plasma, Serum, Supernatant

Ref: IS-I-0400R
Arginine metabolismAmino acidsResearch ELISA kits (RUO)ELISA
DatasheetProtocolMSDS

Arginine (Arg) is a basic non-essential amino acid well known to participate in physiological functions such as i) vascular tone homeostasis as contributing to the production of nitric oxide (NO) and ii) immune response control, through NO synthase (NOS) and Arginase enzymes, respectively.

The Arginine (Arg) ELISA kit allows for the determination of Arg in serum and plasma samples working with a sample volume of 20μL. The kit is easy to implement and well suited for both preclinical and clinical studies.

Sample type Serum, Plasma, Cell culture
Capacity 96 tests
Sensitivity 2.1µM
Range 13 – 500µM
Assay time Sample preparation 3h, ELISA overnight
Reactivity Reacts with all species
References Cited in 3 papers
Size

1 x 96 wells

539.00

68 in stock

Quantity
Total

539.00

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They published with this product  see papers

Yonsei University College of Medicine
University of Manitoba
Gustave Roussy

Product overview

Product name L-Arginine ELISA kit
Description Competitive ELISA kit for the quantitative measurement of Arginine (L-Arg) in serum, plasma and cell culture samples. For research use only
Format 96-well plate
Samples Serum, Plasma, Cell culture
Minimal sample volume 20µL
Reactivity Reacts with all species
Standard range 5.12 – 200µM
Sensitivity

2.1µM

Specificity No significant cross-reactivity was observed with HomoArginine, Agmatine, L-Citruline, L-Ornithine, ADMA and SDMA
Assay time Sample preparation 3h and ELISA overnight
Storage Store at 2-8°C for up to 6 months
Datasheets  Instructions for useMaterial Safety Datasheet

 

Sample collection & storage Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands

Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months)

Sample preparation Sample preparation (3 hours)
ELISA L-Arginine antiserum overnight incubation, revelation and read steps (1h)
Detailed protocol Download instructions for use

Product citations

  • Lactobacillus plantarum ameliorates NASH-related inflammation by upregulating L-arginine production
    Check the article
    Authors : Kim et al., Experimental & Molecular Medicine
    2023-11
  • Physiological and Pathological Considerations for the Use of Flaxseed as a Therapeutic Dietary Strategy
    Check the article
    Authors : Hirst et al., Rev. Cardiovasc. Med.
    2023-05
  • Circulating L-Arginine predicts the survival of cancer patients treated with immune checkpoint inhibitors
    Check the article
    Authors : Peyraud et al., Annals of oncology
    2022-07

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