Arginine ELISA kit – High Sensitivity – Plasma, Serum, Supernatant

Ref: IS-I-0400R
ELISAArginine metabolismAmino acidsResearch ELISA kits (RUO)
DatasheetProtocolMSDS

Arginine (Arg) is a basic non-essential amino acid well known to participate in physiological functions such as i) vascular tone homeostasis as contributing to the production of nitric oxide (NO) and ii) immune response control, through NO synthase (NOS) and Arginase enzymes, respectively.

The Arginine (Arg) ELISA kit allows for the determination of Arg in serum and plasma samples working with a sample volume of 20μL. The kit is easy to implement and well suited for both preclinical and clinical studies.

Sample typeSerum, Plasma, Cell culture
Capacity96 tests
Sensitivity2.1µM
Range13 – 500µM
Assay timeSample preparation 3h, ELISA overnight
ReactivityReacts with all species
ReferencesCited in 2 papers
Size

1 x 96 wells

539.00

71 in stock

Quantity
Total

539.00

Worldwide shipping

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Product overview

Product nameL-Arginine ELISA kit
DescriptionCompetitive ELISA kit for the quantitative measurement of Arginine (L-Arg) in serum, plasma and cell culture samples. For research use only
Format96-well plate
SamplesSerum, Plasma, Cell culture
Minimal sample volume20µL
ReactivityReacts with all species
Standard range5.12 – 200µM
Sensitivity

2.1µM

SpecificityNo significant cross-reactivity was observed with HomoArginine, Agmatine, L-Citruline, L-Ornithine, ADMA and SDMA
Assay timeSample preparation 3h and ELISA overnight
StorageStore at 2-8°C for up to 6 months
Datasheets  Instructions for useMaterial Safety Datasheet

 

Sample collection & storageSerum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands

Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months)

Sample preparationSample preparation (3 hours)
ELISAL-Arginine antiserum overnight incubation, revelation and read steps (1h)
Detailed protocolDownload instructions for use

Product citation

  • Lactobacillus plantarum ameliorates NASH-related inflammation by upregulating L-arginine production
    Check the article
    Authors : Kim et al., Experimental & Molecular Medicine
    2023-11
  • Circulating L-Arginine predicts the survival of cancer patients treated with immune checkpoint inhibitors
    Check the article
    Authors : Peyraud et al., Annals of oncology
    2022-07

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