Antibodies & immunoassays for small molecules D-Serine rabbit pAb – IS1004
Ref: IS1004-sp
NeuromediatorsAmino acidsPolyclonal AntibodiesICCIFIHC
+D-Serine imaging in adult mouse frontal cortex
Using STAINperfect immunostaining kit A and following protocol developped for tissu sections, the anti-D-Serine rabbit polyclonal antibody (Ref: IS1004) revealed the presence of D-Serine postive cells within mouse frontal cortex with a cytoplasmic pattern. The staining was revealed using Alexa Fluor® 488-conjugated secondary antibody and image obtained using confocal imaging.
DatasheetMSDS
The anti-D-Serine antibody IS1004 is a rabbit polyclonal antibody with high affinity and specificity. Combined with the STAINperfect immunostaining kit A, the antibody allows visualizing D-Serine in whole mounts, cell culture and tissue sections.
| Clonality | Polyclonal antibody |
| Host | Rabbit |
| Reactivity | Reacts with all species |
| Eligible samples | Whole mounts, cell culture, tissue sections |
| Staining procedure | STAINperfect immunostaining kit A |
| Format | 50µL (approx. 40 tissue sections) |
Product overview
| Product name | D-Serine antibody – Rabbit pAb |
| Synonyms | Anti-D-Serine antibody Anti (R)-Serine antibody |
| Immunogen | Conjugated D-Serine |
| Specificity | When tested in competitive ELISA, the anti-conjugated D-Serine antibody did not show any significant cross reactivity with D-Serine analogs, including L-Serine and D-Threonine |
| Lot number |
140301 |
Storage
| Form | Liquid |
| Purity | Purified anti-serum |
| Storage buffer | Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Material safety datasheet | Download MSDS |
| IF – Cell cultures, Whole mounts, Tissue sections | Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
| Full protocol | Download STAINperfect protocol for D-Serine staining |
| Protocols-at-a-glance |
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| Complete Instructions for Use |
Protocol-at-a-glance for cell cultures |
Protocol-at-a-glance for whole mounts |
Protocol-at-a-glance for tissue sections |
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Selected publications on D-Serine:
- Paul P, de Belleroche J. The role of D-serine and glycine as co-agonists of NMDA receptors in motor neuron degeneration and amyotrophic lateral sclerosis (ALS). Front Synaptic Neurosci. 2014 Apr 16;6:10.
- Balu DT et al. D-serine and serine racemase are localized to neurons in the adult mouse and human forebrain. Cell Mol Neurobiol. 2014 Apr;34(3):419-35. doi: 10.1007/s10571-014-0027-z. Epub 2014 Jan 17.
- Klatte K et al. Impaired D-serine-mediated cotransmission mediates cognitive dysfunction in epilepsy. J Neurosci. 2013 Aug 7;33(32):13066-80.
- Otte DM et al. Effects of Chronic D-Serine Elevation on Animal Models of Depression and Anxiety-Related Behavior. PLoS One. 2013 Jun 21;8(6):e67131.
- Martineau M et al. Storage and uptake of D-serine into astrocytic synaptic-like vesicles specify gliotransmission. J Neurosci. 2013 Feb 20;33(8):3413-23. doi: 10.1523/JNEUROSCI.3497-12.2013.
- Van Horn MR, Sild M, Ruthazer ES. D-serine as a gliotransmitter and its roles in brain development and disease. Front Cell Neurosci. 2013 Apr 23;7:39. doi: 10.3389/fncel.2013.00039.
