€ 1.049€ 1.198
€ 1.499€ 1.797
€ 1.499€ 1.797
€ 599
Sample type | Any biological sample (including homogenates) |
Capacity | 96-well plate |
Sensitivity | 3.3 pg/mL |
Range | 0.5 – 80 ng/mL |
Assay time | Extraction, acylation and enzymatic conversion (4 hours), ELISA overnight, ELISA revelation & read step (1h) |
Reactivity | Reacts with all species |
Application | Research use only |
The assay works on any biological matrix—plasma, serum, urine, cerebrospinal fluid, culture supernatants or tissue homogenates—and works across all species (dopamine is phylogenetically conserved).
Limit of detection = 3.3 pg mL; calibrated standard curve spans 0.5 – 80 ng mL, allowing accurate measurement from very low to high dopamine levels.
Only 1 µL is sufficient (up to 750 µL can be loaded for dilute samples).
Yes. The kit has already quantified dopamine in culture medium from human mid‑brain organoids at day 70, using just 1 µL per well (Schwamborn lab image in the product gallery). Published studies on Parkinson’s‑disease organoids report similar success — e.g. Zagare 2025 and Muwanigwa 2024. Collect the cleared supernatant or gently homogenise organoids in extraction buffer, then follow the standard acylation → conversion protocol.
Absolutely. It needs only a clear liquid sample, so you can load 1 µL of culture supernatant from your iPSC‑derived neurons after acylation. Fan et al. 2025 used the kit to monitor dopamine released from hydrogel‑grown human dopaminergic neurons with a 3.3 pg mL‑¹ detection limit, capturing both basal and stimulated secretion.
Day 1: extraction + acylation and enzymatic conversion (~4 h). Add antiserum and incubate overnight. Day 2: color development and plate reading (~1 h).
No significant cross‑reactivity was detected with dopamine analogs or related catecholamines, ensuring high specificity.
Yes — quantitative performance has been cross‑validated by LC‑MS, confirming reliability in complex samples.
Keep all components at 2–8 °C; the unopened kit is stable at least six months under these conditions.
This research Dopamine ELISA kit enables the ultra-sensitive measurement of Dopamine (DA) in any biological sample, from any species. Cross-validated by LC-MS, highly flexible, this dopamine assay features a minimal sample volume as low as 1 µL, with a limit of detection at 3.3 pg/mL.
€ 599
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€ 449
€ 1.299€ 1.797
Dopamine ELISA kit – Research test for the ultra-sensitive detection of Dopamine in any sample
Product name | Dopamine ELISA kit – Research |
Description | Enzyme immunoassay (ELISA) for the ultra-sensitive determination of Dopamine in biological samples. Research use only |
Format | 96-well plate |
Samples | Any sample |
Minimal sample volume | 1 – 750 µL |
Reactivity | Reacts with all species |
Standard range | 0.5 – 80 ng/mL |
Sensitivity |
3.3 pg/mL |
Specificity | No significant cross-reactivity was observed with Dopamine analogs. |
Assay time | Sample preparation 4 hours, antiserum incubation overnight, ELISA revelation & read step (1h) |
Storage | Store at 2-8°C for to 6 months |
Sample collection & storage | See instructions for use. |
Sample preparation | Sample extraction and acylation (1h45), enzymatic conversion (2h) |
ELISA | Antisera overnight incubation, revelation and read steps (1h) |