HomoArginine ELISA kit

Ref: IS-I-0500R
Arginine metabolismResearch ELISA kits (RUO)ELISA
DatasheetProtocolMSDS

HomoArginine (hArg) is a basic non-essential amino acid that exerts beneficial effects on vascular homeostasis, more likely through the increase of nitric oxide (NO) that can be explained through two mechanisms since hArg i) serves as a precursor of NO and ii) favors the elevation of L-Arg – main substrate of NO synthase – by inhibiting the enzyme Arginase.

The homoArginine (hArg) ELISA kit allows for the determination of hArg in serum and plasma samples working with a sample volume of 20μL. The kit is easy to implement and well suited for both preclinical and clinical studies.

Sample type Serum, Plasma
Capacity 96 tests
Sensitivity 125nM
Range 0.32 – 12.5µM
Assay time Sample preparation 3h, ELISA overnight
Reactivity Reacts with all species
Size

1 x 96 wells

490.00

100 in stock

Quantity
Total

490.00

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Product overview

Product name HomoArginine ELISA kit
Description Competitive ELISA kit for the quantitative measurement of Homoarginine (hARG) in serum and plasma samples. For research use only
Format 96-well plate
Samples Serum, Plasma
Minimal sample volume 20µL
Reactivity Reacts with all species
Standard range 0.32 – 12.5µM
Sensitivity

125nM

Specificity No significant cross-reactivity was observed with L-Arginine, Agmatine, L-Citruline, L-Ornithine, ADMA and SDMA
Assay time Sample preparation 3h and ELISA overnight
Storage Store at 2-8°C for up to 6 months
Datasheets  Instructions for useMaterial Safety Datasheet

Sample collection & storage Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands

Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months)

Sample preparation Sample preparation (3 hours)
ELISA Homoarginine antiserum overnight incubation, revelation and read steps (1h)
Detailed protocol Download instructions for use

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