L-Asparagine ELISA kit

Ref: IS-I-1600R
Amino acidsResearch ELISA kits (RUO)ELISA
DatasheetProtocolMSDS

L-Asparagine is a conditionally non-essential amino acid that can be produced in our body. However, Asparagine (Asn) is essential for the growth of highly proliferative cells such as cancers and it must be provided by the environment. Targeting Asn using recombinant Asparaginase – as to deprive tumor cells of an important energetic source – thus represents an attractive target and has been for instance approved for the treatment of Acute Lymphoblastic Leukemia.

Also, while targeting Asn metabolism figures as an attractive therapeutic avenue, Asn level constitutes a valuable biomarker. Indeed, among other evidences, it has been shown that quantification of Asn nicely discriminates healthy subjects from patients with Epithelial Ovarian Cancer. More recently, increased Asn level has been linked to poor survival in female patients with colorectal cancer.

 

Sample type Plasma
Capacity 96 tests
Sensitivity 2.3µM (0.30µg/mL)
Range 4.4 – 375µM (0.6 – 49.5µg/mL)
Assay time Sample preparation 3h, ELISA overnight
Reactivity Reacts with all species

 

Size

1 x 96 wells

390.00

Until 06-05-2022

100 in stock

Quantity
Total

390.00

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Product overview

Product name L-Asparagine ELISA kit
Description Competitive ELISA kit for the quantitative measurement of L-Asparagine (Asn) in plasma samples. For research use only
Format 96-well plate
Samples Plasma
Minimal sample volume 20µL
Reactivity Reacts with all species
Standard range 4.4 – 375µM (0.6 – 49.5µg/mL)
Sensitivity

2.3µM (0.30µg/mL)

Specificity No significant cross-reactivity was observed with D-Asparagine, L-Aspartate, L-Glutamate, L-Glutamine.
Assay time Sample preparation 3h and ELISA overnight
Storage Store at 2-8°C for up to 6 months
Datasheets Instructions for useMaterial Safety Datasheet

 

For research use only  – Do not use for diagnostic

Sample collection & storage EDTA Plasma

Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months)

Sample preparation Sample preparation (3 hours)
ELISA L-Asparagine antiserum overnight incubation, revelation and read steps (1h)
Detailed protocol Download instructions for use

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