L-Asparagine ELISA kit

Ref: IS-I-1600R
Amino acidsResearch ELISA kits (RUO)ELISA
DatasheetProtocolMSDS

L-Asparagine is a conditionally non-essential amino acid that can be produced in our body. However, Asparagine (Asn) is essential for the growth of highly proliferative cells such as cancers and it must be provided by the environment. Targeting Asn using recombinant Asparaginase thus represents an attractive target.

In order to provide an accurate and easy-to-implement tool to evaluate L-Asparagine (Asn) functions, we developed and validated an ELISA kit  for Asn quantitation in plasma samples. The assay requires a sample volume as low as 20μL and is characterized by a 2.3µM sensitivity.

Sample type Plasma
Capacity 96 tests
Sensitivity 2.3µM (0.30µg/mL)
Range 4.4 – 375µM (0.6 – 49.5µg/mL)
Assay time Sample preparation 3h, ELISA overnight
Reactivity Reacts with all species

 

Size

1 x 96 wells

490.00

100 in stock

Quantity
Total

490.00

Worldwide shipping

Select delivery location

Product overview

Product name L-Asparagine ELISA kit
Description Competitive ELISA kit for the quantitative measurement of L-Asparagine (Asn) in plasma samples. For research use only
Format 96-well plate
Samples Plasma
Minimal sample volume 20µL
Reactivity Reacts with all species
Standard range 4.4 – 375µM (0.6 – 49.5µg/mL)
Sensitivity

2.3µM (0.30µg/mL)

Specificity No significant cross-reactivity was observed with D-Asparagine, L-Aspartate, L-Glutamate, L-Glutamine.
Assay time Sample preparation 3h and ELISA overnight
Storage Store at 2-8°C for up to 6 months
Datasheets Instructions for useMaterial Safety Datasheet

 

For research use only  – Do not use for diagnostic

Sample collection & storage EDTA Plasma

Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months)

Sample preparation Sample preparation (3 hours)
ELISA L-Asparagine antiserum overnight incubation, revelation and read steps (1h)
Detailed protocol Download instructions for use

You may also like…

L-Glutamine ELISA kit

IS-I-1100R

Sample: 10µl
LoD: 3µM
Standard range: 21 – 3125µM

490.00
VIEW PRODUCTADD
96-w plate format // Low volume samples // Sensitive, easy-to-implement // Cross-validated with LC/MS

L-Serine ELISA kit

IS-I-1200R

Sample: 20µl
LoD: 2.4µM
Standard range: 3.8 – 625µM

490.00
VIEW PRODUCTADD

Arginine ELISA kit

IS-I-0400R

Sample: 20µl
LoD: 2.1µM
Standard range: 5.12 – 200µM

490.00
VIEW PRODUCTADD

L-Phenylalanine ELISA kit

IS-I-1700R

Sample: 20µl
LoD: 1.7µM
Standard range: 3.4 – 390µM

490.00
VIEW PRODUCTADD