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L-Asparagine Antibody – Rabbit Polyclonal
Ref: IS1056Polyclonal AntibodiesICCIFIHCAmino acids
+Affinity & specificity of anti- L-Asparagine antibody
Competitive ELISA demonstrates that low amounts of L-Asparagine conjugate are required to abolish antigen-antibody reaction (high affinity), while rising concentrations of L-Glutamine and L-Aspartate (L-Asparagine competitors) do not affect reaction (high specificity).
DatasheetMSDS
Validated by direct and competitive ELISA, this rabbit polyclonal antibody recognizes L-Asparagine at 1/10 000 working dilution, while displaying no significant cross-reactivity with competitors such as L-Glutamine and L-Aspartate. Combined with the STAINperfect immunostaining kit A, this antibody could allow direct L-Asparagine imaging in various types of samples (cell cultures, tissue sections, whole mounts).
| Clonality | Polyclonal |
| Host | Rabbit |
| Reactivity | Reacts with all species |
| Validated by | ELISA |
| Staining procedure | STAINperfect immunostaining kit A |
| Format | 50µl |
Product overview
| Product name | L-Asparagine – Rabbit pAb |
| Synonyms | Anti-L-Asparagine antibody Anti-(S)-asparagine antibody Anti-Aspartamic acid antibody Anti-L-2,4-diamino-4-oxobutanoic acid antibody |
| Immunogen | Conjugated L-Asparagine |
| Specificity | When tested in competitive ELISA, the anti-conjugated L-Asparagine antibody did not show any significant cross reactivity with L-Ornithine competitors: L-Glutamine and L-Aspartate |
Storage
| Form | Liquid |
| Purity | Purified anti-serum |
| Storage | Store at 4°C |
| Storage buffer | Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Material safety datasheet | Download MSDS |
IF – Cell cultures, Whole mounts, Tissue sections
| Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample | |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
| Full protocol | Download STAINperfect protocol |
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| Complete Instructions for Use | Protocol-at-a-glance for cell cultures | Protocol-at-a-glance for whole mounts | Protocol-at-a-glance for tissue sections |
