Antibodies & immunoassays for small molecules L-Glutamate Polyclonal Antibody
Ref: IS1001
ICCIFIHCNeuromediatorsPolyclonal Antibodies
+Immunostaining of glutamatergic cells in the crayfish brain.
Anti-L-Glutamate antibody highlights the presence of glutamatergic cells into the brain of a crayfish. Staining was obtained with anti-L-Glutamate antibody and performed with STAINperfect immunostaining kit A, following the protocol for whole mounts samples. Alexa Fluor® 488 conjugated secondary antibody was used and images obtained by confocal imaging at high magnification.
DatasheetMSDS
The anti-L-Glutamate antibody IS1001 is a rabbit polyclonal antibody optimized to ensure superior affinity and specificity. Combined with the STAINperfect immunostaining kit A, the antibody allows direct L-Glutamic acid visualization in cell cultures, whole mounts and tissue sections.
| Clonality | Polyclonal antibody |
| Host | Rabbit |
| Reactivity | Reacts with all species |
| Tested samples | Whole mounts, cell culture, tissue sections |
| Staining procedure | STAINperfect immunostaining kit A |
| References | Citations in litterature |
| Format | 50µL (approx. 40 tissue sections) |
Product overview
| Product name | L-Glutamate antibody – Rabbit polyclonal Ab |
| Synonyms | Anti-L-Glutamic acid antibody |
| Immunogen | Conjugated L-Glutamate |
| Specificity | When tested in competitive ELISA, the anti-conjugated L-Glutamate antibody did not show any significant cross reactivity with L-Glutamic acid analogs, including D-Glutamate, L-Glutamine and L-Aspartate conjugates |
| Volume | 50µL |
Storage
| Form | Liquid |
| Purity | Purified anti-serum |
| Storage |
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Material safety datasheet | Download MSDS |
| IF – Cell cultures, Whole mounts, Tissue sections | Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
| Full protocol | Download STAINperfect protocol for L-Glutamate staining |
| Protocols-at-a-glance |
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| Complete Instructions for Use |
Protocol-at-a-glance for cell cultures |
Protocol-at-a-glance for whole mounts |
Protocol-at-a-glance for tissue sections |
Enregistrer
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Product citations:
- Single-cell transcriptomics reveals multiple neuronal cell types in human midbrain-specific organoids
Check the article
Authors : Smits et al., Cell & tissue research
2020-07 - Impact of Sleep-Wake-Associated Neuromodulators and Repetitive Low-Frequency Stimulation on Human iPSC-Derived Neurons
Check the article
Authors : Yokoi et al., Frontiers in neuroscience
2019-05 - hiPS Cell-Derived Neurons for High-Throughput Screening
Check the article
Authors : Traub et al., Methods in molecular biology
2019-01
