L-Kynurenine ELISA kit

Ref: BA-E-2200
Kynurenine pathwayTryptophan metabolismDiagnostic ELISA kits (IVD)ELISA
DatasheetMSDSProtocol

The L-Kynurenine ELISA is an easy-to-use assay tailored for the quantification of Kynurenine across large series of samples. Optimized for the analysis of biological fluids (plasma, cell culture supernatant, serum…), the KYN ELISA features a lower limit of detection at 45.7ng/ml and a minimal sample volume of 20µl, thus allowing longitudinal studies in mice.

Sample type Plasma, serum, cell culture supernatant
Capacity 96 tests
Sensitivity < 45.7ng/ml
Range 0/100 – 10000ng/ml
Assay time Sample acylation 90 min, ELISA overnight
Reactivity Reacts with all species
References Citations in litterature
Size

1 x 96 wells

445.00

100 in stock

Quantity
Total

445.00

Worldwide shipping

Select delivery location

Product overview

Product name L-Kynurenine ELISA kit
Description Enzyme immunoassay (ELISA) for the quantitative determination of L-Kynurenine in biological fluids and cell supernatant
Format 96-well plate
Samples Plasma, serum, cell culture supernatant
Minimal sample volume 20µL
Reactivity Reacts with all species
Standard range 0/100 – 10000ng/ml
Sensitivity

< 47.5ng/ml

Specificity No significant cross-reactivity was observed with L-Kynurenine analogs such as L-Tryptophan, 3-Hydroxy-DL-Kynurenine, 5-Hydroxy-DL-Tryptophan, Tyrosine, Serotonin, Phenylalanin, L-Asparagine and Kynurenic acid
Assay time Sample acylation 90 min and ELISA overnight
Storage Store at 2-8°C for to 6 months
Datasheets Instructions for use, Material Safety Datasheet

 

Kits intended for in-vitro diagnostic (IVD) are not available for the US-market.

In contrast to kits for in-vitro-diagnostic (IVD), kits meant for research use only (RUO) do have a R in the last position of the catalogue number.
The research kit can be ordered with the catalogue no. BA E-2200R.

Sample collection & storage Plasma/Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands. Store smaples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months)
Sample preparation Sample acylation (90 min)
ELISA L-Kynurenine antiserum overnight incubation, revelation and read steps (1h)
Detailed protocol Download instructions for use

  • A highly efficient modality to block the degradation of tryptophan for cancer immunotherapy: locked nucleic acid-modified antisense oligonucleotides to inhibit human indoleamine 2,3-dioxygenase 1/tryptophan 2,3-dioxygenase expression
    Check the article
    Authors : Klar et al., Cancer Immunol Immunother
    02/01/2020
  • Adding Indoximod to Hypofractionated Radiotherapy with Anti-PD-1 Checkpoint Blockade Enhances Early NK and CD8 + T-Cell-Dependent Tumor Activity
    Check the article
    Authors : Watanabe et al., Clinical Cancer Research
    06/11/2019
  • Physical exercise prevents mice from L-Kynurenine-induced depression-like behavior
    Check the article
    Authors : Su et al., Asian Journal of Psychatry
    19/12/2019
  • Exercise training impacts skeletal muscle gene expression related to the kynurenine pathway
    Check the article
    Authors : Allison et al., American Journal of Psycology
    16/01/2019
  • Lactobacillus maintains healthy gut mucosa by producing L-Ornithine
    Check the article
    Authors : Qi et al., Communications Biology
    08/05/2019
  • Leucine competes with kynurenine for blood-to-brain transport and prevents lipopolysaccharide-induced depression-like behavior in mice
    Check the article
    Authors : Walker et al., Molecular Psychiatry
    09/07/2018
  • Dysbiosis of the Vaginal Microbiota and Higher Vaginal Kynurenine/Tryptophan Ratio Reveals an Association with Chlamydia trachomatis Genital Infections
    Check the article
    Authors : Ziklo et al., Frontiers in cellular and infection microbiology
    18/01/2018
  • In vivo inhibition of tryptophan catabolism reorganizes the tuberculoma and augments immune-mediated control of Mycobacterium tuberculosis
    Check the article
    Authors : Gautam et al., Proceedings of the National Academy of Sciences of the USA
    02/01/2018
  • Chronic Mild Stress Alters Kynurenine Pathways Changing the Glutamate Neurotransmission in Frontal Cortex of Rats
    Check the article
    Authors : Martin-Hernandez et al., Molecular Neurobiology
    03/05/2018
  • Use of PD-1 Targeting, Macrophage Infiltration, and IDO Pathway Activation in Sarcomas: A Phase 2 Clinical Trial
    Check the article
    Authors : Toulmonde et al., JAMA oncology
    01/01/2018
  • Inhibition of acute lethal pulmonary inflammation by the IDO-AhR pathway
    Check the article
    Authors : Lee et al., Proceedings of the Nation Academy of Sciences of the USA
    18/07/2017
  • Biological and clinical significance of tryptophan-catabolizing enzymes in cutaneous T-cell lymphomas
    Check the article
    Authors : Maliniemi et al., Oncoimmunology
    10/02/2017
  • The peroxyl radical-induced oxidation of Escherichia coli FtsZ and its single tryptophan mutant (Y222W) modifies specific side-chains, generates protein cross-links and affects biological function
    Check the article
    Authors : Escobar-Alvarez et al., Free radical biology & medicine
    17/07/2017

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