Antibodies & immunoassays for small molecules L-Phenylalanine ELISA kit
Ref: IS-I-1700R
+Typical standard curve Phenylalanine
Typical standard curve obtained with the Phe ELISA kit (#IS-I-1700) where the optical density is inversely correlated with Phe concentration within a range of 3.4 - 390μM.
L-Phenylalanine (Phe) is an essential amino acid that cannot be synthetized by humans or other vertebrates and must be supplied from an exogenous diet. Mutations in the gene encoding for PAH have been linked to Phenyloketonuria (PKU), an aminoacidopathies – associated with fair skin, eczema, seizures, tremors, and hyperactivity – characterized by an increased blood/brain level of Phe and its derivatives, and a concomitant decrease in Tyr.
In order to provide accurate and easy-to-implement tool to better understand Phe functions, we developed and validated a novel ELISA kit which allows for Phe quantitation in biological samples including plasma and sera. The assay requires a sample volume as low as 20μL and is characterized by a 1.7µM sensitivity
| Sample type | Plasma, Serum |
| Capacity | 96 tests |
| Sensitivity | 1.7µM |
| Range | 3.4 – 390µM |
| Assay time | Sample preparation 3h, ELISA overnight |
| Reactivity | Reacts with all species |
Product overview
| Product name | L-Phenylalanine ELISA kit |
| Description | Competitive ELISA kit for the quantitative measurement of L-Phenylalanine (Phe) in plasma and serum samples. For research use only |
| Format | 96-well plate |
| Samples | Plasma, Serum |
| Minimal sample volume | 20µL |
| Reactivity | Reacts with all species |
| Standard range | 3.4 – 390µM |
| Sensitivity |
1.7µM |
| Specificity | No significant cross-reactivity was observed with L-Tyrosine, L-DOPA, Dopamine hydrochloride or Noradrenaline |
| Assay time | Sample preparation 3h and ELISA overnight |
| Storage | Store at 2-8°C for up to 6 months |
| Datasheets | Instructions for use, Material Safety Datasheet |
For research use only – Do not use for diagnostic
| Sample collection & storage | EDTA Plasma
Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months) |
| Sample preparation | Sample preparation (3 hours) |
| ELISA | L-Phenylalanine antiserum overnight incubation, revelation and read steps (1h) |
| Detailed protocol | Download instructions for use |
Selected articles on Phenylalanine:
- Atila et al., The serum amino-acid profile in COVID-19, Amino Acids, 2021
