Antibodies & immunoassays for small molecules L-Phenylalanine ELISA kit
Ref: IS-I-1700R
+Typical standard curve Phenylalanine
Typical standard curve obtained with the Phe ELISA kit (#IS-I-1700) where the optical density is inversely correlated with Phe concentration within a range of 3.4 - 390μM.
L-Phenylalanine (Phe) is an essential amino acid that cannot be synthetized by humans or other vertebrates and must be supplied from an exogenous diet. Phe is transformed to L-Tyrosine (Tyr) through the Phenylalanine hydroxylase (PAH) enzyme and thus serves as a precursor of key catecholamines including Dopamine, Noradrenaline and Adrenaline. Mutations in the gene encoding for PAH have been linked to Phenyloketonuria (PKU), an aminoacidopathies – associated with fair skin, eczema, seizures, tremors, and hyperactivity – characterized by an increased blood/brain level of Phe and its derivatives, and a concomitant decrease in Tyr. Besides its known role in PKU, Phe has been recently linked to severity of COVID-19 where higher level of Phe has been detected for patients with moderate/severe disease in comparison to patients with asymptomatomatic/midly SARS-COV-2 infection (Atila et al, Amino Acids, 2021) thus shedding light on role of Phe on the control of immune response.
| Sample type | Plasma, Serum |
| Capacity | 96 tests |
| Sensitivity | 1.7µM |
| Range | 3.4 – 390µM |
| Assay time | Sample preparation 3h, ELISA overnight |
| Reactivity | Reacts with all species |
Product overview
| Product name | L-Phenylalanine ELISA kit |
| Description | Competitive ELISA kit for the quantitative measurement of L-Phenylalanine (Phe) in plasma and serum samples. For research use only |
| Format | 96-well plate |
| Samples | Plasma, Serum |
| Minimal sample volume | 20µL |
| Reactivity | Reacts with all species |
| Standard range | 3.4 – 390µM |
| Sensitivity |
1.7µM |
| Specificity | No significant cross-reactivity was observed with L-Tyrosine, L-DOPA, Dopamine hydrochloride or Noradrenaline |
| Assay time | Sample preparation 3h and ELISA overnight |
| Storage | Store at 2-8°C for up to 6 months |
| Datasheets | Instructions for use, Material Safety Datasheet |
For research use only – Do not use for diagnostic
| Sample collection & storage | EDTA Plasma
Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months) |
| Sample preparation | Sample preparation (3 hours) |
| ELISA | L-Phenylalanine antiserum overnight incubation, revelation and read steps (1h) |
| Detailed protocol | Download instructions for use |
Selected articles on Phenylalanine:
- Atila et al., The serum amino-acid profile in COVID-19, Amino Acids, 2021
