L-Phenylalanine ELISA kit

Ref: IS-I-1700R
Amino acidsResearch ELISA kits (RUO)ELISA
DatasheetProtocolMSDS

L-Phenylalanine (Phe) is an essential amino acid that cannot be synthetized by humans or other vertebrates and must be supplied from an exogenous diet. Phe is transformed to L-Tyrosine (Tyr) through the Phenylalanine hydroxylase (PAH) enzyme and thus serves as a precursor of key catecholamines including Dopamine, Noradrenaline and Adrenaline. Mutations in the gene encoding for PAH have been linked to Phenyloketonuria (PKU), an aminoacidopathies – associated with fair skin, eczema, seizures, tremors, and hyperactivity – characterized by an increased blood/brain level of Phe and its derivatives, and a concomitant decrease in Tyr. Besides its known role in PKU, Phe has been recently linked to severity of COVID-19 where higher level of Phe has been detected for patients with moderate/severe disease in comparison to patients with asymptomatomatic/midly SARS-COV-2 infection (Atila et al, Amino Acids, 2021) thus shedding light on role of Phe on the control of immune response.

Sample type Plasma, Serum
Capacity 96 tests
Sensitivity 1.7µM
Range 3.4 – 390µM
Assay time Sample preparation 3h, ELISA overnight
Reactivity Reacts with all species
Size

1 x 96 wells

490.00

100 in stock

Quantity
Total

490.00

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Product overview

Product name L-Phenylalanine ELISA kit
Description Competitive ELISA kit for the quantitative measurement of L-Phenylalanine (Phe) in plasma and serum samples. For research use only
Format 96-well plate
Samples Plasma, Serum
Minimal sample volume 20µL
Reactivity Reacts with all species
Standard range 3.4 – 390µM
Sensitivity

1.7µM

Specificity No significant cross-reactivity was observed with L-Tyrosine, L-DOPA, Dopamine hydrochloride or Noradrenaline
Assay time Sample preparation 3h and ELISA overnight
Storage Store at 2-8°C for up to 6 months
Datasheets Instructions for useMaterial Safety Datasheet

 

For research use only  – Do not use for diagnostic

Sample collection & storage EDTA Plasma

Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months)

Sample preparation Sample preparation (3 hours)
ELISA L-Phenylalanine antiserum overnight incubation, revelation and read steps (1h)
Detailed protocol Download instructions for use

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