L-Phenylalanine ELISA kit I High Sensitivity I Plasma, Serum

Ref: IS-I-1700R
Amino acidsResearch ELISA kits (RUO)ELISA
DatasheetProtocolMSDS

L-Phenylalanine (Phe) is an essential amino acid that cannot be synthetized by humans or other vertebrates and must be supplied from an exogenous diet. Mutations in the gene encoding for PAH have been linked to Phenyloketonuria (PKU), an aminoacidopathies – associated with fair skin, eczema, seizures, tremors, and hyperactivity – characterized by an increased blood/brain level of Phe and its derivatives, and a concomitant decrease in Tyr.

In order to provide accurate and easy-to-implement tool to better understand Phe functions, we developed and validated a novel ELISA kit which allows for Phe quantitation in biological samples including plasma and sera. The assay requires a sample volume as low as 20μL and is characterized by a 1.7µM sensitivity

Sample typePlasma, Serum
Capacity96 tests
Sensitivity1.7µM
Range3.4 – 390µM
Assay timeSample preparation 3h, ELISA overnight
ReactivityReacts with all species
Size

1 x 96 wells

539.00

95 in stock

Quantity
Total

539.00

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Product overview

Product nameL-Phenylalanine ELISA kit
DescriptionCompetitive ELISA kit for the quantitative measurement of L-Phenylalanine (Phe) in plasma and serum samples. For research use only
Format96-well plate
SamplesPlasma, Serum
Minimal sample volume20µL
ReactivityReacts with all species
Standard range3.4 – 390µM
Sensitivity

1.7µM

SpecificityNo significant cross-reactivity was observed with L-Tyrosine, L-DOPA, Dopamine hydrochloride or Noradrenaline
Assay timeSample preparation 3h and ELISA overnight
StorageStore at 2-8°C for up to 6 months
DatasheetsInstructions for useMaterial Safety Datasheet

 

For research use only  – Do not use for diagnostic

Sample collection & storageEDTA Plasma

Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months)

Sample preparationSample preparation (3 hours)
ELISAL-Phenylalanine antiserum overnight incubation, revelation and read steps (1h)
Detailed protocolDownload instructions for use

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