Antibodies & immunoassays for small molecules Quinolinic Acid ELISA kit
Ref: IS-I-0100R
Kynurenine pathwayTryptophan metabolismResearch ELISA kits (RUO)ELISA
+Quinolinic acid (QA) is a metabolite produced along the Kynurenine Pathway
Quinolinic acid (QA) is a metabolite produced along the Kynurenine Pathway, which converts the aminoacid Tryptophan to NAD+, a co-factor of many enzymatic reactions. It has been described to share several neurotoxic functions particularly through the activation of the N-Methyl-D-Aspartate receptor. Its production occurring in myeloid cells (macrophages, microglia, etc.) is mainly driven by in ammatory stress and is therefore suspected to play a key role in neuroin ammatory disorders such as Alzheimer and Parkinson diseases but also Multiple Sclerosis, Amyotrophic Lateral Scle- rosis. Hence, while QA has been proposed as a biomarker in these indications, it might certainly warrant further investigation to serve as a surro- gate in other “in ammation” related diseases such as cancer, metabolic disorders, etc.
DatasheetMSDSProtocol
Quinolinic acid (QA) is a metabolite produced along the Kynurenine Pathway, which converts the aminoacid Tryptophan to NAD+, a co-factor of many enzymatic reactions.
The Quinolinic Acid ELISA kit is optimized for the quantitation of Quinolinic Acid (QUIN or QA) within serum and plasma samples. This easy-to-use competitive assay is well-suited for both small and large series of samples. Working with a low sample volume of 25µL, this assay enables pre-clinical and clinical sample testings.
| Sample type | Serum, Plasma |
| Capacity | 96 tests |
| Sensitivity | < 6ng/mL |
| Range | 25 – 2000ng/mL |
| Assay time | Sample acylation 120 min, ELISA overnight |
| Reactivity | Reacts with all species |
Product overview
| Product name | Quinolinic Acid ELISA kit |
| Description | Competitive ELISA kit for the quantitative measurement of Quinolinic Acid levels in serum and plasma samples. For research use only |
| Format | 96-well plate |
| Samples | Serum, Plasma |
| Minimal sample volume | 25µL |
| Reactivity | Reacts with all species |
| Standard range | 25 – 2000ng/mL |
| Sensitivity |
< 6ng/mL |
| Specificity | No significant cross-reactivity was observed with Quinolinic Acid analogs such as Kynurenic acid, Xanthurenic acid, Kynurenine, Picolinic acid, 3-Hydroxy-Anthranilic Acid |
| Assay time | Sample acylation 120 min and ELISA overnight |
| Storage | Store at 2-8°C for up to 6 months |
| Datasheets | Instructions for use, Material Safety Datasheet |
| Sample collection & storage | Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands
Plasma (Heparin) Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months) |
| Sample preparation | Sample acylation (120 min) |
| ELISA | Quinolinic acid antiserum overnight incubation, revelation and read steps (1h) |
| Detailed protocol | Download instructions for use |
Selected articles on Quinolinic acid
- Sundaram et al. Quinolinic acid toxicity on oligodendroglial cells: relevance for multiple sclerosis and therapeutic strategies. J Neuroinflammation. 2014 Dec 13
