Any species, any biological matrix – plasma, serum, CSF, cell or tissue lysate and urine. Matrix effects are minimized by the acetylation step.

Yes – several papers report serotonin quantification in Drosophila melanogaster larvae and adults, in Aedes aegypti mosquitoes, and in C. elegans nematodes. These studies confirm that direct homogenisation of small model organisms followed by the standard acetylation step yields reliable data.

Rinse the organism briefly in cold PBS, blot dry, then homogenise (10–20 µL ice‑cold PBS + 0.1 % ascorbic acid per mg wet weight). Centrifuge 10 min / 4 °C / 10 000 g and use 1 µL supernatant per well, diluting further if values exceed the 2.5 ng/mL top standard. The 5 pg/mL LOD and wide 0.015–2.5 ng/mL range allow substantial dilution head‑room.

Absolutely. Mouse cortical and hippocampal tissue, zebrafish brain extracts and multiple rodent memory‑model studies all cite robust serotonin recovery using the kit’s standard protocol.

Begin with a 1 : 20 to 1 : 50 (w/v) homogenate in assay buffer, then load the undiluted supernatant. Most rodent brains give 0.1–1 ng/mg, which places readings mid‑curve; if OD values rise above the top calibrator, dilute the supernatant up to 1 : 200. The kit’s sensitivity (5 pg/mL) means even highly diluted brain samples remain well within range.

5 pg / mL (0.005 ng / mL) as determined by the kit standard curve.

As little as 1 µL per well.

Yes – the antibody reacts with serotonin irrespective of species origin.

No significant cross‑reactivity was observed with tryptamine, melatonin, 5‑HIAA or related indoles.

Prep 30 min, overnight incubation, read in 1 h next day – hands‑on <2 h.

Standard microplate reader capable of measuring 450 nm absorbance, plus pipettes and a shaker.

96‑well precoated plate, serotonin standards, detection antibody, conjugate, substrate, stop solution, buffers, acetylation reagent, IFU.

2–8 °C; shelf‑life 6 months from delivery.