Antibodies & immunoassays for small molecules Xanthurenic acid Monoclonal Antibody
Ref: IS009
Kynurenine pathwayMonoclonal AntibodiesICCIFIHC
+Xanthurenic acid detection by IF in human midbrain
Xanthurenic acid visualization by immunohistofluorescence in human caudate putamen. Staining highlights cytoplasmic accumulation of Xanthurenic acid in brain cells. Paraffin-embedded brain tissue section was subjected to pH=9 antigen retrieval followed by overnight incubation with primary antibody (dilution 1/500). After incubation with Alexa-488 conjugated secondary Ab, epifluorescence microscopy (100X) was used to capture the staining.
DatasheetMSDS
The first and only validated anti-Xanthurenic acid antibody available for research use. Internal validation for IHC and IF of the 6C12-A12 mAb highlighted cytoplasmic presence of xanthurenic acid in human midbrain tissues.
| Clonality | Monoclonal antibody (clone 6C12-A12) |
| Host | Mouse |
| Valided applications | IHC / IF |
| Reactivity | Reacts with all species |
| References | Not yet cited to our knowledge Submit content and get a 10% discount! |
| Format | 50µL |
Product overview
| Product name | Xanthurenic acid antibody |
| Synonyms | Anti- Xanthurenic acid antibody
8-Hydroxy-4-oxo-1H-quinoline-2-carboxylic acid antibody Xanthuric acid antibody Xanthurenate antibody 8-Hydroxykynurenic acid antibody |
| Immunogen | Conjugated Xanthurenic acid |
| Clone | clone 6C12-A12 |
| Specificity | When tested in competitive ELISA, the anti-conjugated Xanthurenic acid antibody did not show any significant cross-reactivity with competitors Kynurenic acid and Quinaldic acid conujugates |
Storage
| Form | Liquid |
| Purity | Purified IgG |
| Concentration | 0,5mg/ml |
| Storage |
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Material safety datasheet | Download MSDS |
| Immunohistochemistry (IHC) | Dilute at 1:200-1:2000. Perform heat antigen retrieval (pH=9) before initiating IHC staining protocol on paraffin-embedded and frozen sections |
| Immunofluorescence (IF) | Dilute at 1:100-1:1000 on paraffin-embedded and frozen sections. Perform heat antigen retrieval and incubate with fluorescent secondary antibody conjugate |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
Antibody not yet cited. Submit an article and get a 10% discount.
Selected publications on Xanthurenic acid
- Neale SA, Copeland CS, Salt TE.Effect of VGLUT inhibitors on glutamatergic synaptic transmission in the rodent hippocampus and prefrontal cortex.Neurochem Int. 2013 Oct 10. pii: S0197-0186(13)00250-7. doi: 10.1016/j.neuint.2013.10.001. [Epub ahead of print]
- Neale SA, Copeland CS, Uebele VN, Thomson FJ, Salt TE. Modulation of hippocampal synaptic transmission by the kynurenine pathway member xanthurenic acid and other VGLUT inhibitors. Neuropsychopharmacology. 2013 May;38(6):1060-7. doi: 10.1038/npp.2013.4. Epub 2013 Jan 7.
- Taleb O, Maammar M, Brumaru D, Bourguignon JJ, Schmitt M, Klein C, Kemmel V, Maitre M, Mensah-Nyagan AG.Xanthurenic acid binds to neuronal G-protein-coupled receptors that secondarily activate cationic channels in the cell line NCB-20.PLoS One. 2012;7(11):e48553. doi: 10.1371/journal.pone.0048553. Epub 2012 Nov 6.
- Copeland CS1, Neale SA, Salt TE.Actions of Xanthurenic acid, a putative endogenous Group II metabotropic glutamate receptor agonist, on sensory transmission in the thalamus.Neuropharmacology. 2013 Mar;66:133-42. doi: 10.1016/j.neuropharm.2012.03.009. Epub 2012 Apr 2.
