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Dopamine ELISA kit ultra sensitive any sample BA-E-5300R

Quantification of dopamine release in cell supernatantMeasurement of dopamine release in the supernatant of human midbrain organoids (wild-type or SNCA triplication) at day 70, using the Dopamine BA-E-5300R ELISA kit. Each data point represents four independent batches (pooling media from 3 organoid wells). Courtesy of Schwamborn lab.

Key features

Sample type	Any biological sample (including homogenates)
Capacity	96-well plate
Sensitivity	3.3 pg/mL
Range	0.5 – 80 ng/mL
Assay time	Extraction, acylation and enzymatic conversion (4 hours), ELISA overnight, ELISA revelation & read step (1h)
Reactivity	Reacts with all species
Application	Research use only

Citations

2025 Krasley et.al., - Molecular Determinants of Optical Modulation in ssDNA-Carbon Nanotube Biosensors: Insights from Experimental and Computational Approaches, ACS Nano, Species : ; Sample type : Solution ;
2025 Fan et al., - Establishment of a novel method for differentiating into dopaminergic neurons using charged hydrogels, Biochem Biophys Res Commun., Species : Human ; Sample type : Cell culture supernatant ;
2025 Zagare A. et al., - Insulin resistance compromises midbrain organoid neuronal activity and metabolic efficiency predisposing to Parkinson’s disease pathology, J Tissue Eng., Species : Human ; Sample type : Organoid ;
2024 Muwanigwa MN et al., - Alpha-synuclein pathology is associated with astrocyte senescence in a midbrain organoid model of familial Parkinson’s disease, Molecular and Cellular Neuroscience, Species : Human ; Sample type : Organoid ;
2024 Matsuda T et al., - Striatal parvalbumin interneurons, not cholinergic interneurons, are activated in a mouse model of cerebellar dystonia, Dis Model Mech, Species : Mouse ; Sample type : Brain ;
2024 Hur K.H. et al., - Transient receptor potential ankyrin 1 channel modulates the abuse-related mechanisms of methamphetamine through interaction with dopamine transporter, British Journal of Pharmacology, Species : Mouse ; Sample type : Brain ;
2024 Sasaki et al., - Rosemary extract activates oligodendrogenesis genes in mouse brain and improves learning and memory ability, Biomed Pharmacother , Species : Mouse ; Sample type : Brain ;
2024 Xu et al., - Placenta-derived SOD3 deletion impairs maternal behavior via alterations in FGF/FGFR-prolactin signaling axis, Cell Reports, Species : Mouse ; Sample type : Brain ;
2024 Inoue et al., - Lineage-specific duplication and functional diversification of DOPA-decarboxylase genes in the Gryllidae family, as revealed in Gryllus bimaculatus, Insect Biochem Mol Biol, Species : Insects ; Sample type : Brain ;
2023 Kurlawala et al., - Fine Particulate Matter (PM2.5)-Induced Pulmonary Oxidative Stress Contributes to Changes in the Plasma Lipidome and Liver Transcriptome in Mice, Toxicological Sciences, Species : Mouse ; Sample type : Plasma ;
2023 Fujino et al., - Dopamine is involved in reparative dentin formation through odontoblastic differentiation of dental pulp stem cells, Scientific Reports, Species : Human ; Sample type : Cell culture supernatant ;
2023 Nagano et al., - An Alternative Method for Quantitative Mass Spectrometry Imaging (q-MSI) of Dopamine Utilizing Fragments of Animal Tissue, Mass spectrometry, Species : Mouse ; Sample type : Brain ;
2023 Hwang et al., - Adinazolam, a Benzodiazepine-Type New Psychoactive Substance, Has Abuse Potential and Induces Withdrawal Symptoms in Rodents, ACS Chemical Neurosciences, Species : Rat ; Sample type : Brain ;
2023 Aktar S. et al., - Transcriptomics and biochemical evidence of trigonelline ameliorating learning and memory decline in the senescence-accelerated mouse prone 8 (SAMP8) model by suppressing proinflammatory cytokines and elevating neurotransmitter release, GeroScience , Species : Mouse ; Sample type : Brain ;
2023 Somuah-Asante et al., - Behavioral and Biochemical Evaluation of Anti-Depressive and Oxidative Stress-Ameliorating Effects of Amber Extract in Adult Male ICR Mice, Nutraceuticals, Species : Mouse ; Sample type : Brain ;
2022 Honke et al., - Endogenously produced catecholamines improve the regulatory function of TLR9-activated B cells, Plos Biology, Species : Mouse ; Sample type : Spleen ;
2022 Abbondanza et al., - Nicotinic acetylcholine receptors expressed by striatal interneurons inhibit striatal activity and control striatal-dependent behaviors, The J of Neuroscience, Species : Mouse ; Sample type : Brain ;
2022 Uefune et al., - Dopamine Negatively Regulates Insulin Secretion Through Activation of D1-D2 Receptor Heteromer, Diabetes, Species : Mouse ; Sample type : Pancreas ;
2022 Hur K-H et al., - Mepirapim, a Novel Synthetic Cannabinoid, Induces Addiction-Related Behaviors through Neurochemical Maladaptation in the Brain of Rodents, Pharmaceuticals, Species : Rat ; Sample type : Brain ;
2022 Sasaki et al., - Microalgae extract induces antidepressant-like activity via neuroinflammation regulation and enhances the neurotransmitter system, Food and chemical Toxicology, Species : Mouse ; Sample type : Brain ;
2022 Wauquier et al., - Circulating Human Serum Metabolites Derived from the Intake of a Saffron Extract (Safr’InsideTM) Protect Neurons from Oxidative Stress: Consideration for Depressive Disorders, Nutrients, Species : Human ; Sample type : Cell culture supernatant ;
2022 Sudevan et al., - Loss of physical contact in space alters the dopamine system in C. elegans, iScience, Species : C. Elegans ; Sample type : Whole animal ;
2021 Okimura et al., - Photoperiodic regulation of dopamine signaling regulates seasonal changes in retinal photosensitivity in mice, Scientific reports, Species : Mouse ; Sample type : Eye ;
2021 Omari et al., - Dietary administration of cumin-derived cuminaldehyde induce neuroprotective and learning and memory enhancement effects to aging mice, Aging, Species : Mouse ; Sample type : Brain ;
2021 Bekal et al., - Neurobehavioral effects of acute low-dose whole-body irradiation, J of Radiation Research, Species : Mouse ; Sample type : Blood ;
2021 Sasaki K et al., - Grape skin extract modulates neuronal stem cell proliferation and improves spatial learning in senescence-accelerated prone 8 mice, Aging, Species : Mouse ; Sample type : Brain ;
2021 Miyazaki et al., - Ninjin’yoeito, a traditional Japanese medicine, increases dopamine content in PC12 cells, Bioscience, Biotechnology, and Biochemistry, Species : Rat ; Sample type : Cell culture supernatant ;
2021 Sasaki et al., - Antidepressant- and anxiolytic-like activities of Rosmarinus officinalis extract in rodent models: Involvement of oxytocinergic system, Biomedicine & Pharmacotherapy, Species : Mouse ; Sample type : Brain ;
2021 Achour et al., - Luteolin Modulates Neural Stem Cells Fate Determination: In vitro Study on Human Neural Stem Cells, and in vivo Study on LPS-Induced Depression Mice Model, Frontiers in cell and developmental biology, Species : Mouse ; Sample type : Brain ;
2021 Makizaki et al., - Improvement of loperamide-induced slow transit constipation by Bifidobacterium bifidum G9-1 is mediated by the correction of butyrate production and neurotransmitter profile due to improvement in dysbiosis, PLoS One, Species : Rat ; Sample type : Serum ;
2020 Shimizu et al., - Novel Drosophila model for parkinsonism by targeting phosphoglycerate kinase, Neurochemistry International , Species : Insects ; Sample type : Drosophila heads ;
2020 Iwata et al., - Sugarcane (Saccharum officinarum L.) Top Extract Ameliorates Cognitive Decline in Senescence Model SAMP8 Mice: Modulation of Neural Development and Energy Metabolism, Frontiers in Cell and Developmental Biology, Species : Mouse ; Sample type : Brain ;
2019 Sabti et al., - Elucidation of the Molecular Mechanism Underlying Lippia citriodora(Lim.)-Induced Relaxation and Anti-Depression, International Journal of Molecular Sciences, Species : Mouse ; Sample type : Brain ;
2019 Sasaki et al., - Antidepressant-Like Effect of Ferulic Acid via Promotion of Energy Metabolism Activity, Mol. Nutr. Food Res., Species : Mouse ; Sample type : Brain ;
2019 Golomidov et al., - The neuroprotective effect of fullerenols on a model of Parkinson’s disease in Drosophila melanogaster, Biochemical and Biophysical Research Communications, Species : Insects ; Sample type : Drosophila heads ;

Frequently asked questions

BA-E-5300R

Dopamine ELISA kit – Any sample

This research Dopamine ELISA kit enables the ultra-sensitive measurement of Dopamine (DA) in any biological sample, from any species. Cross-validated by LC-MS, highly flexible, this dopamine assay features a minimal sample volume as low as 1 µL, with a limit of detection at 3.3 pg/mL.

€599

Number of citations :

73 in stock

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For questions, protocol recommendations, and troubleshooting, you have direct access to the scientist that developed the antibody or ELISA kit you’re using.

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BA-E-5300R

Dopamine ELISA kit – Any sample

Dopamine ELISA kit – Research test for the ultra-sensitive detection of Dopamine in any sample

Specifications

Product name	Dopamine ELISA kit – Research
Description	Enzyme immunoassay (ELISA) for the ultra-sensitive determination of Dopamine in biological samples. Research use only
Format	96-well plate
Samples	Any sample
Minimal sample volume	1 – 750 µL
Reactivity	Reacts with all species
Standard range	0.5 – 80 ng/mL
Sensitivity	3.3 pg/mL
Specificity	No significant cross-reactivity was observed with Dopamine analogs.
Assay time	Sample preparation 4 hours, antiserum incubation overnight, ELISA revelation & read step (1h)

Instructions

Storage	Store at 2-8°C for to 6 months
Sample collection & storage	See instructions for use.
Sample preparation	Sample extraction and acylation (1h45), enzymatic conversion (2h)
ELISA	Antisera overnight incubation, revelation and read steps (1h)

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