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L-DOPA Antibody – Rabbit Polyclonal
Ref: IS1030IHCNeuromediatorsCatecholaminesPolyclonal AntibodiesICCIF
+Imaging of L-DOPA neurons in mouse primary neurons
Our rabbit polyclonal anti-L-DOPA antibody - IS1030 - allows the detection of L-DOPA neurons in primary mouse neurons. Staining was performed using STAINperfect immunostaining kit A, according to the protocol optimized for cell culture. Fluorescent labeled secondary antibody was used and picture was acquired by high-content imaging. Such staining highlights the presence of L-DOPA both in soma and neurons fibers.
DatasheetMSDS
The anti-L-DOPA antibody IS1030 is a rabbit polyclonal antibody validated for IF in crayfish tissues and mouse primary neurons. Following optimal sample preparation with the STAINperfect immunostaining kit A, our antibody directly stains L-DOPA in whole mounts, cell culture, and tissue sections.
| Clonality | Polyclonal antibody |
| Host | Rabbit |
| Reactivity | Reacts with all species |
| Tested samples | Whole mounts, cell culture, tissue sections |
| Staining procedure | STAINperfect immunostaining kit A |
| Format | 50µL (approx. 40 tissue sections) |
Product overview
| Product name | L-DOPA antibody – Rabbit pAb |
| Synonyms | Anti-3,4-Dihydroxy-L-phenylalanine antibody |
| Immunogen | Conjugated L-DOPA |
| Specificity | When tested in competitive ELISA, the anti-conjugated L-DOPA antibody did not show any significant cross reactivity with L-Serine analogs, including Normetanephrine and Dopamine conjugates |
Storage
| Form | Liquid |
| Purity | Purified anti-serum |
| Storage buffer | Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Material safety datasheet | Download MSDS |
| IF – Cell cultures, Whole mounts, Tissue sections | Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
| Full protocol | Download STAINperfect protocol for L-DOPA staining |
| Protocols-at-a-glance |
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| Complete Instructions for Use | Protocol-at-a-glance for cell cultures | Protocol-at-a-glance for whole mounts | Protocol-at-a-glance for tissue sections |
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