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Typical standard curve of 3-Hydroxy-Kynurenine-ELISAExample of standard curve obtained with the 3-HK ELISA Kit. Because the assay is competitive, optical density decreases as 3-Hydroxy-Kynurenine concentration increases (inverse correlation).
Cross-validation 3-Hydroxy-Kynurenine ELISA vs LC-MSHuman serum samples (n=33) were processed for 3-Hydroxy-Kynurenine measurement by mean of both 3-HK ELISA kit and liquid chromatography–mass spectrometry (LC/MS). As depicted, results obtained with ELISA and LC/MS are highly correlated (R Squared=0.9328).
Key features
| Sample type | EDTA plasma |
| Capacity | 96-well plate |
| Sensitivity | 3.8 nM |
| Range | 10 – 390.65 nM |
| Assay time | Sample precipitation & derivatization 2.5h, ELISA overnight |
| Reactivity | Reacts with all species |
| Application | Research use only |
Frequently asked questions
3-Hydroxy-Kynurenine ELISA kit
The 3-Hydroxy-Kynurenine (3-HK) ELISA Kit provides a simple, highly sensitive method for quantifying 3-HK in just 50 µL of plasma from any species. Cross-validated against LC-MS in human plasma, the kit is well-suited for in vitro, preclinical, and clinical studies.
This kit is especially valuable for research into neurodegenerative and neuroinflammatory conditions—where neurotoxic, pro-oxidant 3-HK accumulates—such as Alzheimer’s, Huntington’s, and Parkinson’s diseases, as well as in major depressive disorder and schizophrenia.
€ 599
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3-Hydroxy-Kynurenine ELISA kit
3-hydroxy-kynurenine ELISA ELISA kit – Research test for the detection of 3-HK in plasma samples
Specifications
| Product name | 3-Hydroxy-Kynurenine ELISA |
| Description | Competitive ELISA kit for the quantitative measurement of 3-Hydroxy-Kynurenine ELISA in plasma samples. For research use only |
| Format | 96-well plate |
| Samples | EDTA plasma |
| Minimal sample volume | 50µL |
| Reactivity | Reacts with all species |
| Standard range | 10 – 390.65 nM |
| Sensitivity | 3.8 nM |
| Specificity | No significant cross-reactivity was observed with Kynurenine, Kynurenic acid, 3-Hydorxy-Anthranilic Acid, Xanthurenic Acid, Tryptophan. |
| Assay time | Sample precipitation & derivatization 2.5h, ELISA overnight |
Instructions
| Storage | Store at 2-8°C for to 6 months |
| Sample collection & storage | EDTA Plasma – store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months) |
| Sample preparation | Sample precipitation & derivatization (2.5h) |
| ELISA | 3-HK antiserum overnight incubation, revelation, and read steps (1h) |
