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Anthranilic acid detection by IHC in human brainImmunohistochemical analysis of human caudate-putamen highlights Anthranilic acid accumulation in the cytoplasm of glial cells. Paraffin-embedded brain tissue was subjected to pH=6 antigen retrieval followed by overnight incubation with primary antibody (dilution 1/50). After incubation with polymer conjugated secondary Ab, DAB was used to reveal the staining.
Affinity & specificity of anti-Anthranilic acid antibodyCompetitive ELISA demonstrates that low amounts of Anthranilic acid conjugate are required to abolish antigen-antibody reaction (high affinity), while rising concentrations of 3-OH-Anthranilic acid conjugate do not affect reaction (high specificity).
Anthranilic acidTryptophan catabolism along the kynurenine pathway has been extensively investigated over the past decades for its roles in immune regulation and neurotransmission. Anthranilic acid is synthesized directly from L-kynurenine, to produce 3-hydroxyanthranilic acid without generation of neurotoxic 3-OH-Kynurenine. Recently, Anthranilic acid has been described in organismal death of C.elegans, as well as in pathogen Pseudomonas aeruginosa's tryptophan catabolism.
Key features
| Clonality | Monoclonal antibody |
| Host | Mouse |
| Reactivity | Reacts with all species |
| Tested samples | Human FFPE tissue sections (brain) |
| Staining procedure | Perform heat antigen retrieval (pH=6) before standard IHF/IF staining |
| Format | 50µL (approx. 40 tissue sections) |
Frequently asked questions
Anthranilic acid Antibody – Mouse Monoclonal
The monoclonal anti-Anthranilic acid antibody 10G1-F1 was validated for immunohistochemistry (IHC) in human brain tissues. Highly specific, as demonstrated by competitive ELISA, the antibody consistently delivered clean and reproducible stainings
€449
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IS013
Anthranilic acid Antibody – Mouse Monoclonal
Anti-Anthranilic acid mAb – Purified monoclonal antibody for research use only (liquid 50µL)
Specifications
| Product name | Anthranilic acid antibody |
| Synonyms | 2-AA antibody
Anti- vitamin L1 antibody Anti- Anthranilate antibody Anthranilate antibody 2-Aminobenzoic acidantibody |
| Immunogen | Conjugated Anthranilic acid |
| Isotype |
IgG1 k chain |
| Clone |
Clone 10G1-F1 |
| Specificity | When tested in competitive ELISA, the anti-anthranilic antibody did not show any significant cross reactivity with 3-hydroxyAnthranilic acid |
| Volume | 50 µL |
| Form | Liquid |
| Purity | Purified IgG |
Instructions
| Storage |
Store at +4°C for short term (6 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Immunohistochemistry (IHC) | Dilute at 1:50-1:500. Perform heat antigen retrieval (pH=6) before initiating IHC staining protocol on paraffin-embedded and frozen sections |
| Immunofluorescence (IF) |
Dilute at 1:50-1:500 on paraffin-embedded and frozen sections. Perform heat antigen retrieval and incubate with fluorescent secondary antibody conjugate |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
