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Typical standard curve of Citrulline ELISATypical standard curve obtained with the CIT ELISA kit. In this competitive enzyme immunoassay, optical density is inversely correlated with CIT levels within a linear range of 6,4 – 250 μM. (example data - do not use for calculation
Cross-validation of L-Citrulline ELISA and LC/MS data in human plasma samplesCIT was quantified in plasma samples from 40 human subjects either using IS I-1500R ELISA kit or by liquid chromatography–mass spectrometry (LC/MS). Correlation coefficient of 0,9932 confirms the accuracy of the immunoassay.
Key features
| Sample type | Plasma |
| Capacity | 96-well plate |
| Sensitivity | 1,47 μM |
| Range | 6,4 – 250 μM |
| Assay time | Sample extraction (2.5h), ELISA overnight |
| Reactivity | Reacts with all species |
| Application | Research use only |
Frequently asked questions
Citrulline ELISA kit
The Citrulline ELISA kit allows easy and accurate quantification of L-citrulline in plasma samples. Plasma citrulline (CIT) can serve as a biomarker to assess intestinal health (enterocyte function) and disorders related to the urea cycle and nitric oxide biosynthesis (see our L-Arginine ELISA kit). With a minimal sample volume of 20 µL and a detection limit of 1.47 μM, the kit enables working with samples of human and animal origin.
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For questions, protocol recommendations, and troubleshooting, you have direct access to the scientist that developed the antibody or ELISA kit you’re using.
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A knowledge base containing protocols, Frequently Asked Questions, and troubleshooting tips is also available online.
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IS I-1500R
Citrulline ELISA kit
L-Citrulline ELISA kit – Research test for the detection of L-Citrulline
Specifications
| Product name | L-Citrulline ELISA |
| Description | Competitive ELISA kit for the quantitative measurement of L-Citrulline in plasma samples. For research use only |
| Format | 96-well plate |
| Samples | Plasma |
| Minimal sample volume | 20µL |
| Reactivity | Reacts with all species |
| Standard range | 6,4 – 250 μM |
| Sensitivity |
1,47 μM |
| Specificity | No significant cross-reactivity was observed with L-Critulline analogs |
| Assay time | Sample extraction 2.5h, ELISA overnight |
Instructions
| Storage | Store at 2-8°C for to 6 months |
| Sample collection & storage | EDTA or Heparin plasma samples only. Do not use haemolytic, icteric or lipemic samples. Storage: up to 24 hours at 2 – 8°C; for longer periods (up to 3 months) at – 20°C |
| Sample preparation | Sample preparation, precipitation, derivatization (2,5h) |
| ELISA | Citrulline antiserum incubation (overnight), revelation and read steps (1h) |
