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Key features
| Sample type | Plasma, serum |
| Capacity | 2 x 96 tests |
| Sensitivity | ARG: 2.1µM CIT: 1.47µM |
| Range | ARG: 5.12 – 200µM CIT: 6.4 – 250µM |
| Assay time | Sample preparation (3h), ELISA overnight |
| Reactivity | Reacts with all species |
| Application | Research use only |
Frequently asked questions
Citrulline-to-Arginine ratio ELISA pack
Measuring the Citrulline-to-Arginine ratio is indicative of nitric oxide synthase (NOS) activity, which converts Arginine into Citrulline and Nitric Oxide (NO). A decreased plasma CIT-to-ARG ratio thus suggests high NO production, and may serve as a biomarker in metabolic and cardiovascular conditions.
€1049€1198
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For questions, protocol recommendations, and troubleshooting, you have direct access to the scientist that developed the antibody or ELISA kit you’re using.
Direct customer support is available all business days.
A knowledge base containing protocols, Frequently Asked Questions, and troubleshooting tips is also available online.
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ISE-0510R
Citrulline-to-Arginine ratio ELISA pack
ELISA pack composed of :
– IS-I-0400R – Arginine ELISA kit – Research test for the detection of L-Arginine
– IS-I-1500R – Citrulline ELISA kit – Research test for the detection of L-Citrulline
Specifications
| Product name | Citrulline-to-Arginine ELISA pack |
| Description | Two enzyme immunoassays (ELISA) allowing the quantitative determination of L-Citrulline and L-Arginine in plasma/serum samples. For research use only |
| Format | 2 x 96-well plates |
| Samples | Plasma, serum |
| Minimal sample volume | 20µL |
| Reactivity | Reacts with all species |
| Standard range | ARG: 5.12 – 200µM CIT: 6.4 – 250µM |
| Sensitivity | ARG: 2.1µM CIT: 1.47µM |
| Specificity | No significant cross-reactivity was observed with analogs for each kit. See product pages for Arginine ELISA and Citrulline ELISA |
| Assay time | Sample preparation (3h), ELISA overnight |
Instructions
| Storage | Store at 2-8°C for to 6 months |
| Sample collection & storage | Plasma: Use EDTA as anticoagulantSerum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands.
Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months) |
| Sample preparation | Sample preparation (3h) |
| ELISA | Antisera overnight incubation, revelation and read steps (1h) |
