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Key features
| Sample type | Serum, plasma |
| Capacity | 96-well plate |
| Sensitivity | 7.74 ng/mL |
| Range | 0/20 – 800 ng/mL |
| Assay time | 75 min |
| Reactivity | Any species |
| Application | Research use or in vitro diagnostics (IVD in EU only) |
Frequently asked questions
Cortisol ELISA kit – Serum and Plasma
This rapid ELISA kit quantitatively measures cortisol, the primary glucocorticoid in humans, in serum and plasma samples. Measuring stress hormone cortisol is crucial for assessing endocrine function (HPA axis), stress response, circadian rhythm, and disorders related to adrenal insufficiency. Designed for both in vitro diagnostic and research use, its 96-well plate format facilitates high-throughput testing and ensures fast turnaround times.
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Direct Customer Support
For questions, protocol recommendations, and troubleshooting, you have direct access to the scientist that developed the antibody or ELISA kit you’re using.
Direct customer support is available all business days.
A knowledge base containing protocols, Frequently Asked Questions, and troubleshooting tips is also available online.
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Cortisol ELISA kit – Serum and Plasma
Enzyme immunoassay for the quantitative measurement of cortisol in serum and plasma
Specifications
| Product name | Cortisol ELISA |
| Description | In vitro diagnostics / Research enzyme immunoassay (ELISA) for the quantitative determination of Cortisol in serum and plasma |
| Format | 96-well plate |
| Samples | Serum, plasma |
| Minimal sample volume | 20µL |
| Reactivity | Any species |
| Standard range | 0/20 – 800 ng/mL |
| Sensitivity | 7.74 ng/mL |
| Specificity | No significant cross-reactivity was observed with cortisol analogs such as Corticosterone, Cortisone, Deoxycortisol, Dexamethazone, Estrone, Progesterone and Testosterone. |
| Assay time | 75 min |
Instructions
| Storage | Store at 2-8°C for to 6 months |
| Sample collection & storage | Serum or plasma (EDTA, heparin or citrate plasma): Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months). |
| Sample preparation | Enzymatic conjugation (60 min) |
| ELISA | Incubation, revelation and read steps (15 min) |
