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Dopaminergic and serotoninergic neurons in crayfish brainImmunostaining of dopaminergic and serotoninergic brain neurons of a crayfish. Staining was performed with STAINperfect immunostaining kit A, following the protocol for whole mount samples. Fluorescent secondary antibodies were used and pictures were acquired by confocal imaging with high magnification.
Dopamine (green), TH (white), Hoechst (blue) in 30-day brain organoid (hiPSC)Courtesy Sarah Nickels from the Schwamborn lab
Dopaminergic neurons of the Substantia Nigra pars compacta (SNc) in coronal rat brain sectionsRevealed by anti-dopamine (DA, rabbit polyclonal antibody #IS1005) and Tyrosine Hydroxylase (TH, #MAB5280, reference catecholaminergic neuron immunostaining). As highlighted in the overlay, DA immunoreactivity well correlates with TH immunoreactivity in the SNc, thus showing that our antibody against the DA neurotransmitter - used with the STAINperfect immunostaining kit A – is a validated tool to directly highlight DAergic systems rather than through biosynthesis enzyme immunostaining, expressed in all catecholaminergic cell populations.
Dopamine and Serotonin immunostaining in the CNS of embryonic mouseUsing STAINperfect immunostaining kit A, Dopamine (green) and Serotonin (red) were stained in the CNS of embryonic mouse E13.5. Staining were obtained following the protocol optimized for whole mount using IS1005 rabbit polyclonal antibody against Dopamine and IS0135 goat polyclonal antibody against Serotonin. Fluorescent conjugated secondary antibodies were then used and images captured by confocal microscopy at high magnification.
Dopamine (3,4-dihydroxyphenethylamine)Dopamine (DA) is a catecholamine neurotransmitter and hormone synthesized from L-DOPA. Playing a key role in motor, motivational and cognitive functions, brain dopaminergic systems are found to be altered in a number of pathological states, including Parkinson's disease, attention deficit hyperactivity disorder (ADHD), drug addictions, pain and behavioral disorders. At the peripheral level, dopamine is an important regulator of blood flow, which also exerts paracrine and exocrine effects on immune cells, renal proximal tubular cells and as well as pancreatic cells.
Immunostaining of Dopamine and Serotonin in mouse culture of primary midbrain neuronsDopamine and Serotonin were stained in mouse culture of primary midbrain neurons using STAINperfect immunostaining kit A. Staining were obtained following the protocol optimized for cell culture using IS1005 rabbit polyclonal antibody against Dopamine and IS0135 goat polyclonal antibody against Serotonin. Fluorescent conjugated secondary antibodies were then used and imaged by confocal imaging.
Key features
| Clonality | Polyclonal antibody |
| Host | Rabbit |
| Reactivity | Reacts with all species |
| Tested samples | Rodent primary neurons & tissues, human cell cultures & iPS-derived brain organoids, crayfish (whole mount), C. Elegans (whole animal) |
| Staining procedure | STAINperfect immunostaining kit A (protocols for cell cultures, whole mounts, tissue sections) |
| Format | 50µL (approx. 40 tissue sections) |
Frequently asked questions
IS1005
Dopamine Antibody – Rabbit Polyclonal
The anti-dopamine antibody IS1005 was used in recent papers to visualize dopamine in mouse primary neurons, rodent & human brain tissues, human iPS-derived brain organoids, and whole animals (C. Elegans). Combined with the STAINperfect immunostaining kit A, this rabbit polyclonal antibody enables high-quality dopamine imaging in cell cultures, whole mounts and tissue sections from any species.
€449
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IS1005
Dopamine Antibody – Rabbit Polyclonal
Anti-Dopamine pAb – Purified polyclonal antibody for research use only (liquid)
Specifications
| Product name | Dopamine antibody – Rabbit polyclonal Ab |
| Synonyms | Anti-Dopamine antibody Anti-3,4-dihydroxyphenethylamine antibody Anti-DA antibody Anti-hydroxytyramine antibody Anti-oxytyramine |
| Immunogen | Conjugated Dopamine |
| Specificity | When tested in competitive ELISA, the anti-conjugated Dopamine antibody did not show any significant cross reactivity with Tyramine and L-Dopa conjugates |
| Volume | 50 µL |
| Form | Liquid |
| Purity | Purified anti-serum |
Instructions
| Storage |
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Immunofluorescence (IF) |
Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample (Cell cultures, Whole mounts, Tissue sections) |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
