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Total and GABAergic neuronal networks in mature rat cortical culturesImage field micrographs of mature (12 DIV-old) rat cortical cultures, illustrating total and GABAergic neuronal networks revealed by MAP2 (in red) and GABA (in green) immunostaining, respectively. GABA immunostaining obtained using whether our monoclonal (#IS039) or polyclonal GABA antibody (#IS1006) with the STAINperfect immunostaining
GABAergic network and glutamatergic cell populations in a mature rat cortical cultureGABAergic network and glutamatergic cell populations in a mature rat cortical culture revealed by GABA (mouse monoclonal antibody #IS039) and L-glutamate (rabbit polyclonal antibody #IS1001) immunoreactivity. As expected, our antibodies, appropriately used with the STAINperfect immunostaining kit A, highlight the rich cortical GABAergic system (cell bodies and branches - displaying a punctiform staining) as well as the glutamate-positive cell population. While GABA neurons are also glutamate-positive (some are pointed by the arrows), arrowheads point glutamate-positive GABA-negative cells, which can be either astrocytes or glutamatergic neurons.
GABAergic neuronal networkin a mature rat cortical culture revealed through GABA immunoreactivity detection using our mouse monoclonal GABA antibody (#IS039) with the STAINperfect immunostaining kit A.
Affinity & specificity of anti-GABA antibodyCompetitive ELISA demonstrates that low amounts of GABA conjugate are required to abolish antigen-antibody reaction (high affinity), while rising concentrations of beta-Alanine and L.Glutamate conjugates do not affect reaction (high specificity).
Gamma-aminobutyric acid (GABA)In the mammalian brain, inhibitory neurotransmitter Gamma-aminobutyric acid (GABA) is mainly synthesized from excitatory L-Glutamate by enzyme glutamic acid decarboxylase (GAD). Regulating neuronal excitability, GABAergic synapses are present throughout the CNS, although GABA is found most highly concentrated in the subtantia nigra, the globus pallidus nuclei, the hypothalamus, the periaqueductal grey matter and the hyppocampus.
Key features
| Clonality | Monoclonal antibody |
| Host | Mouse |
| Reactivity | Reacts with all species |
| Tested samples | Primary rat neurons, Human midbrain organoids |
| Staining procedure | STAINperfect immunostaining kit A (protocols for cell cultures, whole mounts, tissue sections) |
| Format | 50µL (approx. 40 tissue sections) |
Frequently asked questions
GABA Antibody – Mouse Monoclonal
Used with the STAINperfect immunostaining kit A, the anti-GABA antibody IS039 allows to label Gamma-Aminobutyric acid in cell cultures, whole mounts and tissue sections, evidencing GABAergic systems (cell bodies and neuritic network). We successfully performed GABA stainings in rat primary neurons with this antibody, and a recent publication used this anti-GABA antibody in human iPS-derived midbrain organoids. Our range also includes polyclonal anti-GABA antibodies: Rabbit pAb #IS1006, Chicken pAb#IS1036.
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IS039
GABA Antibody – Mouse Monoclonal
Anti-GABA mAb – Purified monoclonal antibody for research use only (liquid 50µL)
Specifications
| Product name | GABA antibody – mouse mAb |
| Synonyms | Anti-g-Aminobutyric acid antibodyAnti-gamma-Aminobutyric acid antibody |
| Immunogen | Conjugated GABA |
| Isotype |
IgG1 k chain |
| Clone |
Clone 7F52B41B4 |
| Specificity | When tested in competitive ELISA, the anti-conjugated GABA antibody did not show any significant cross reactivity with gamma-Aminobutyric acid analogs, including beta-Alanine and L-Glutamate |
| Volume | 50 µL |
| Form | Liquid |
| Purity | Purified IgG |
Instructions
| Storage |
Store at +4°C for short term (6 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Immunofluorescence (IF) |
Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample (Cell cultures, Whole mounts, Tissue sections) |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
