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5-HT -antibody Immunolabeling of Octopaminergic and Serotoninergic neuronsImmunostaining of crayfish eyetalk using anti-octopamine rabbit polyclonal antibody (green) and anti-serotonin goat polyclonal antibody (red). Tissus were processed with whole mount protocol of STAINperfect immunostaining kit A. Fluorescent labeled secondary antibodies were used and pictures were acquired by confocal imaging with high magnification.
Dopamine (green) and Serotonin (red) immunostaining in mouse culture of primary midbrain neuronsDopamine and Serotonin were stained in mouse culture of primary midbrain neurons. Stainings were obtained following the STAINperfect immunostaining kit A protocol optimized for cell culture using IS1005 rabbit polyclonal antibody against Dopamine and IS0135 goat polyclonal antibody against Serotonin. Fluorescent conjugated secondary antibodies were then used and images acquired using confocal imaging.
Dopamine (green) and Serotonin (red) immunostaining in the CNS of embryonic mouse E13.5Dopamine (green) and Serotonin (red) were stained in the CNS of embryonic mouse E13.5 following the protocol optimized for whole mount of the STAINperfect immunostaining kit A. IS1005 rabbit polyclonal antibody against Dopamine and IS0135 goat polyclonal antibody against Serotonin were used. Fluorescent conjugated secondary antibodies were then used and images captured by confocal imaging.
Serotoninergic neurons in crayfish brainLow magnification of serotoninergic neurons in the brain of a crayfish. This staining shows serotonin presence in soma and fibers. Tissue was processed following whole mount protocol of STAINperfect immunostaining kit A. Fluorescent labeled secondary antibody was used and image was obtained by fluorescence imaging.
Serotonin in crayfish eyetalkHigh magnification of serotoninergic neurons in a crayfish's eyetalk. This staining shows serotonin presence in soma containing vesicules and projections. Tissue was processed according to whole mount protocol of STAINperfect immunostaining kit A. Fluorescent labeled secondary antibody was used and picture was captured by confocal imaging.
Affinity & specificity of anti-Serotonin antibodyCompetitive ELISA demonstrates that low amounts of Serotonin conjugate are required to abolish antigen-antibody reaction (high affinity), while rising concentrations of Serotonin competitors Tryptamine or 5-MethoxyTryptamine do not affect reaction (high specificity).
Key features
| Clonality | Polyclonal antibody |
| Host | Rabbit |
| Reactivity | Reacts with all species |
| Tested samples | Mouse primary neurons and brain tissues, crayfish tissues |
| Staining procedure | STAINperfect immunostaining kit A (protocols for cell cultures, whole mounts, tissue sections) |
| Format | 50µL (approx. 40 tissue sections) |
Frequently asked questions
What is the Goat Anti-Serotonin Antibody – Polyclonal (IS1035)?
A purified goat-derived polyclonal antibody targeting serotonin (5-HT), designed for sensitive and specific labeling in research applications.
Which species and sample types has this antibody been validated on?
Validated across all species, including mouse primary neurons, brain tissues, and crayfish samples.
What staining techniques is it compatible with?
Optimized for use with the STAINperfect™ immunostaining kit A, supporting protocols for cell cultures, whole mounts, and tissue sections.
What format and volume does the product come in?
Comes as a liquid - 50 µL per vial—sufficient for around 40 tissue sections, depending on application.
What is its specificity profile?
Exhibits high affinity and high specificity: in competitive ELISA assays, serotonin conjugate effectively competes, whereas structurally related analogs (e.g., tryptamine, 5-methoxytryptamine) show no significant interference.
What are the recommended storage conditions?
Short-term: +4 °C (1–2 months) Long-term: –20 °C, ideally aliquoted to avoid freeze–thaw cycles
How should the antibody be used in immunofluorescence (IF)?
- Use with STAINperfect kit A’s antibody diluent - Recommended dilution range: 1:250 to 1:1000 - Follow the STAINperfect protocol tailored to your sample type - Fine-tune dilutions for optimal signal based on your system
Is this antibody approved for diagnostic or therapeutic use?
No—This product is strictly for research use only.
Are supporting documents and protocols available?
Yes. Downloadable datasheet, MSDS, and detailed STAINperfect protocols (for cell cultures, whole mounts, tissue sections) are available on the product page.
Goat Anti-Serotonin Antibody – Polyclonal
The goat Anti-Serotonin Antibody (IS1035) provides sensitive and specific detection of serotonin (5-HT). Validated in mouse neurons, brain tissues, and crayfish models using the STAINperfect™ immunostaining kit, it ensures robust and reliable serotonin labeling for neuroscience research.
€ 449
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For questions, protocol recommendations, and troubleshooting, you have direct access to the scientist that developed the antibody or ELISA kit you’re using.
Direct customer support is available all business days.
A knowledge base containing protocols, Frequently Asked Questions, and troubleshooting tips is also available online.
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IS1035
Goat Anti-Serotonin Antibody – Polyclonal
Anti-Serotonin pAb – Purified polyclonal antibody for research use only (liquid 50µL)
Specifications
| Product name | Serotonin antibody (5-HT goat polyclonal Ab) |
| Synonyms | Anti-5-HT antibodyAnti-5-hydroxytryptamine antibody Anti-hydroxytryptamine antibody |
| Immunogen | Conjugated 5-HT |
| Specificity | When tested in competitive ELISA, the anti-conjugated Serotonin antibody did not show any significant cross reactivity with 5-Hydroxytryptamine analogs, including Tryptamine and 5-HydroxyTryptophan |
| Volume | 50 µL |
| Form | Liquid |
| Purity | Purified anti-serum |
Instructions
| Storage |
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Immunofluorescence (IF) |
Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample (Cell cultures, Whole mounts, Tissue sections) |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
