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Immunofluorescence imaging of Histamine in mouse midbrainHistamine was detected into whole mount mouse midbrain using STAINperfect immunostaining kit A. Staining was obtained following the protocol for whole mounts samples preparation combined with our IS1039 rabbit polyclonal anti-histamine antibody. After incubation with a fluorescent labeled secondary antibody, confocal imaging was performed for picture acquisition.
Histamine detection into crayfish CNSUsing STAINperfect immunostaining kit A, Histamine was detected into the CNS of a crayfish. Staining was obtained following the protocol for whole mounts samples with IS1039 rabbit polyclonal anti-histamine antibody. Fluorescent labeled secondary antibody was used and picture was acquired by confocal imaging.
Key features
| Clonality | Polyclonal antibody |
| Host | Rabbit |
| Reactivity | Reacts with all species |
| Tested samples | Mouse & crayfish brain tissues |
| Staining procedure | STAINperfect immunostaining kit A (protocols for cell cultures, whole mounts, tissue sections) |
| Format | 50µL (approx. 40 tissue sections) |
Frequently asked questions
Histamine Antibody – Rabbit Polyclonal
The anti-histamine antibody IS1039 is a rabbit polyclonal antibody developed to label Histamine in whole mounts, cell culture and tissue sections. The antibody was validated for IF in mouse and crayfish brain tissues, using the STAINperfect immunostaining kit A.
€449
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For questions, protocol recommendations, and troubleshooting, you have direct access to the scientist that developed the antibody or ELISA kit you’re using.
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IS1039
Histamine Antibody – Rabbit Polyclonal
Anti-Histamine pAb – Purified polyclonal antibody for research use only (liquid 50µL)
Specifications
| Product name | Histamine antibody – Rabbit pAb |
| Synonyms | Anti-Histamine antibody Anti-2-(4-Imidazolyl)ethylamine antibody |
| Immunogen | Conjugated Histamine |
| Specificity | When tested in competitive ELISA, the anti-conjugated Histamine antibody did not show any significant cross reactivity with Histidine conjugate |
| Volume | 50 µL |
| Form | Liquid |
| Purity | Purified anti-serum |
Instructions
| Storage |
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Immunofluorescence (IF) |
Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample (Cell cultures, Whole mounts, Tissue sections) |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
