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Key features
| Sample type | Any sample |
| Capacity | 96-well plate |
| Sensitivity | 0.2 ng/mL |
| Range | 0.5 – 50 ng/mL |
| Assay time | Sample acylation (90 min), incubation (3h or overnight), ELISA revelation & read steps (90 min) |
| Reactivity | Reacts with all species |
| Application | Research use only |
Frequently asked questions
Histamine ELISA kit – Any sample
This research Histamine ELISA kit enables the ultra-sensitive measurement of histamine in any biological sample: tissue homogenates, cell culture supernatants, body fluids, etc. With a minimal sample volume as low as 10µl, this histamine immunoassay allows longitudinal studies in mice and rat models.
€599
100 in stock
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BA-E-5800R
Histamine ELISA kit – Any sample
Histamine ELISA kit – Ultra-sensitive research test for the detection of histamine
Specifications
| Product name | Histamine ELISA kit – Any sample – Research |
| Description | Enzyme immunoassay (ELISA) for the quantitative determination of histamine levels in biological samples. Research use only |
| Format | 96-well plate |
| Samples | Any sample, including homogenates |
| Minimal sample volume | 10 µL |
| Reactivity | Reacts with all species |
| Standard range | 0 / 0.5 – 50 ng/mL |
| Sensitivity |
0.2 ng/mL |
| Specificity | No significant cross-reactivity was observed with Histamine analogs such as 3-Methyl-Histamine, Tyramine, L-Phenylalanine, L-Histidine, L-Tyrosine, Tryptamine, 5-Hydroxy-Indole-Acetic Acid, Serotonin. |
| Assay time | Sample acetylation (90 min) and ELISA overnight |
Instructions
| Storage | Store at 2-8°C for to 6 months |
| Sample collection & storage | See instructions for use |
| Sample preparation | Sample predilution and acylation (1h30) |
| ELISA | Histamine antiserum incubation (3h or overnight), revelation and read steps (1h30) |
