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Kynurenic-Acid-Quinolinic-Acid-ELISA-ratio-pack-5Kynurenic acid (KYNA) and quinolinic acid (QA) are downstream metabolites of the kynurenine pathway with well‑established neuromodulatory and immunomodulatory activities. Quantifying KYNA and QA in biological samples is increasingly investigated as a potential biomarker strategy for various diseases.
Measurement of plasma levels of both KYNA and QA in mice treated with KMO inhibitors.Mice were administered one of two selective KMO inhibitors—Ro‑618048 or UPF648—at two dose levels. Plasma samples were collected before dosing and at 1.5 h and 6 h post‑treatment. Kynurenic acid and quinolinic acid concentrations were then quantified using ELISA kits IS‑I‑0200 and IS‑I‑0100.
KYNA / QA ELISA standard curvesExamples of standard curves obtained with the Kynurenic acid and Quinolinic acid ELISA kits. Because these assays are competitive, the optical density (OD) decreases as KYNA or QA concentration increases. The curves are shown for illustration only and must not be used for quantitative calculations.
KYNA and QA along the Kynurenine PathwayKynurenic acid (KYNA) and quinolinic acid (QA) are downstream metabolites of the kynurenine pathway with well‑established neuromodulatory and immunomodulatory activities. Quantifying KYNA and QA in biological samples is increasingly proposed as a biomarker strategy for a range of disorders.
Key features
| Sample type | Serum, Plasma |
| Capacity | 96 tests |
| Sensitivity | LoD KYNA: 0.53ng/mL
LoD QA: 6ng/mL |
| Range | KYNA: 1.40 – 74ng/mL
QA: 25 – 200ng/mL |
| Assay time | Sample preparation (90-120 min), ELISA overnight |
| Reactivity | Reacts with all species |
| Application | Research use only |
Frequently asked questions
Kynurenic Acid/Quinolinic Acid ELISA pack
Immusmol’s two ready‑to‑use ELISA kits enable the quantification of kynurenic acid (KYNA) and quinolinic acid (QA) in serum or plasma. Requiring only a minimal sample volume (50µL) and compatible with any species, the assays fit seamlessly into both pre‑clinical and clinical workflows.
These kits have already helped researchers investigate neuroinflammatory disorders such as bipolar disorder and multiple sclerosis in human plasma and serum samples.
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For questions, protocol recommendations, and troubleshooting, you have direct access to the scientist that developed the antibody or ELISA kit you’re using.
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A knowledge base containing protocols, Frequently Asked Questions, and troubleshooting tips is also available online.
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ISE-0102R
Kynurenic Acid/Quinolinic Acid ELISA pack
Specifications
| Product name | Kynurenic Acid/Quinolinic Acid ELISA pack |
| Description | Two enzyme immunoassays (ELISA) allowing the quantitative determination of Kynurenic acid and Quinolinic acid in serum samples. For research use only |
| Format | 2x 96-well plates |
| Samples | Serum, Plasma |
| Minimal sample volume | 25-50µL |
| Reactivity | Reacts with all species |
| Standard range | KYNA: 1.40 – 74ng/ml
QA: 25 – 2000ng/ml |
| Sensitivity |
LoD KYNA: 0.53ng/mL LoD QA: 6ng/mL |
| Specificity | No significant cross-reactivity was observed with analogs for each kit. See product pages for Kynurenic acid ELISA and Quinolinic acid ELISA |
| Assay time | Sample preparation 3h and ELISA overnight |
Instructions
| Storage | Store at 2-8°C for up to 6 months |
| Sample collection & storage | Serum: Do not use lipemic, haemolytic samples, or samples containing precipitates or fibrin strands.
Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months) |
| Sample preparation | Sample preparation (90-120 min) |
| ELISA | antiserum overnight incubation, revelation and read steps (1h) |
