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Staining of glutamatergic neurons in mouse primary cortical cultureOur optimized mouse monoclonal anti-L-Glutamate antibody allows the detection of L-Glutamate within primary neurons in cell culture. Staining was performed using STAINperfect immunostaining kit A, according to the protocol optimized for cell culture. After addition of a fluorescent labeled
L-Glutamate and GABA in adult mouse primary cortical neuronsAdult mouse primary cortical neurons were stained with mouse monoclonal anti-L-glutamate antibody (red) combined with anti-GABA rabbit polyclonal antibody (green). Staining was performed using optimized sample preparation with STAINperfect immunostaining kit A and according to the protocol for cell culture. Fluorescent labeled secondary antibody were used and pictures were acquired by confocal imaging.
L-Glutamic acid (L-Glutamate)Amino acid L-Glutamic acid (L-Glutamate) is the major excitatory neurotransmitter in the vertebrate nervous system. Agonist of NMDA, AMPA, Kainate and metabotropic receptors, L-Glutamic acid regulates synaptic plasticity, and is thus involved in learning and mnemonic processes. However, by activating NMDA receptors, L-Glutamic acid may also lead to neuronal damage and death. Glutamate toxicity is thus associated with the pathogenesis of neurodevelopmental and neurodegenerative disorders.
Affinity & specificity of anti-L-Glutamate monoclonal antibody 1D5-1B1Competitive ELISA demonstrates that low amounts of L-Glu conjugate are required to abolish antigen-antibody reaction (high affinity), while rising concentrations of D-Glutamate and L-Aspartate conjugates do not affect reaction (high specificity).
Key features
| Clonality | Monoclonal antibody |
| Host | Mouse |
| Reactivity | Reacts with all species |
| Tested samples | Mouse primary neurons, human iPSC-derived neurons |
| Staining procedure | STAINperfect immunostaining kit A (protocols for cell cultures, whole mounts, tissue sections) |
| Format | 50µL (approx. 40 tissue sections) |
Frequently asked questions
L-Glutamate Antibody – Mouse Monoclonal
The anti-L-Glutamate antibody IS018 was validated in mouse primary neurons in combination with the STAINperfect immunostaining kit A. A recent paper also used this mouse monoclonal antibody to stain for L-Glutamate in human iPS-derived neurons.
€449
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IS018
L-Glutamate Antibody – Mouse Monoclonal
Anti-L-Glutamate mAb – Purified monoclonal antibody for research use only (liquid 50µL)
Specifications
| Product name | L-Glutamate antibody – mouse mAb |
| Synonyms | Anti-L-Glutamic acid antibody |
| Immunogen | Conjugated L-Glutamate |
| Isotype |
IgG1 k chain |
| Clone |
Clone 1D51B1 |
| Specificity | When tested in competitive ELISA, the anti-conjugated Glutamate antibody did not show any significant cross reactivity with its analogs, including D-Glutamate |
| Volume | 50 µL |
| Form | Liquid |
| Purity | Purified IgG |
Instructions
| Storage |
Store at +4°C for short term (6 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Immunofluorescence (IF) |
Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample (Cell cultures, Whole mounts, Tissue sections) |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
