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Key features
| Sample type | Plasma |
| Capacity | 96-well plate |
| Sensitivity | 1.4µM |
| Range | 10 – 390µM |
| Assay time | Sample preparation 3h, ELISA overnight |
| Reactivity | Reacts with all species |
| Application | Research use only |
Frequently asked questions
L-Histidine ELISA kit
The IS-I-1300R ELISA kit enables the sensitive and quantitative measurement of L-Histidine (His) in plasma samples from any species. Cross-validated against LC-MS in human plasma, the assay offers high sensitivity and requires only 10 µL of sample, making it fit for both preclinical research and clinical studies.
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IS-I-1300R
L-Histidine ELISA kit
L-Histidine ELISA kit – Research test for the detection of L-Histidine (His)
Specifications
| Product name | L-Histidine ELISA kit |
| Description | Competitive ELISA kit for the quantitative measurement of L-Histidine (His) in plasma samples. For research use only |
| Format | 96-well plate |
| Samples | Plasma |
| Minimal sample volume | 10µL |
| Reactivity | Reacts with all species |
| Standard range | 10 – 390 µM |
| Sensitivity |
1.4µM |
| Specificity | No significant cross-reactivity was observed with Histamine dihydrochloride, L-Glutamic acid, 3-Methyl-L-histidine & L-Histidinol dihydrochloride |
| Assay time | Sample preparation 3h and ELISA overnight |
Instructions
| Storage | Store at 2-8°C for to 6 months |
| Sample collection & storage | EDTA Plasma – store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months) |
| Sample preparation | Sample preparation (3 hours) |
| ELISA | L-Histidine antiserum overnight incubation, revelation and read steps (1h) |
