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L-kynurenine ELISA kit assay immusmol BA-E-2200

Determination of L-Kynurenine levels in cell cultures through microdialysis collection and by ELISA quantificationMurine CT26 colorectal cancer cell line WT or stably transfected with vector containing cDNA encoding for indoleamine 2,3 dioxygenase (IDO1) or Human U-87 MG glioblastoma cell line were cultured for 48 hours. Cell culture was subjected to microdialysis collection (3 fractions collected) and kynurenine was measured using the Kynurenine ELISA kit (BA-E-2200). As expected, kynurenine level was higher in IDO1-proficient CT26 cells than in parental (WT) CT26 cell lines, and also high in U87-MG cells.

Kynurenine ELISA cross-validation by GC/MS in human plasma samplesCitrate-anticoagulated human plasma samples were processed for Kynurenine measurement by gas-chromatography/mass-spectrometry and ELISA. As shown here, ELISA and GC/MS-based results showed a high correlation.

Determination of L-Kynurenine levels in CT26 cell cultures by ELISADetermination of L-Kynurenine levels in cell cultures through microdialysis collection and by ELISA quantification

L-Kynurenine quantification by ELISA in 20µl mice plasma samples

Kynurenine ELISA – typical standard curveExample of standard curve obtained with the Kynurenine ELISA Kit. In this competitive ELISA, optical density is invertly correlated with Kynurenine levels. Only serves as example - do not use for calculation.

$Murine CT26 colorectal cancer cell line WT or stably transfected with vector containing cDNA encoding for indoleamine 2,3 dioxygenase (IDO1) or Human U-87 MG glioblastoma cell line were cultured for 48 hours. Cell culture was subjected to microdialysis collection (3 fractions collected) and kynurenine was measured using the Kynurenine ELISA kit (BA-E-2200). As expected, kynurenine level was higher in IDO1-proficient CT26 cells than in parental (WT) CT26 cell lines, and also high in U87-MG cells.$

Key features

Sample type	Plasma, serum, cell culture supernatant
Capacity	96-well plate
Sensitivity	LoD: 47.5ng/mL
Range	100 – 10,000 ng/ml
Assay time	Sample acetylation (90 min), overnight incubation, ELISA revelation & read step (1h)
Reactivity	Reacts with all species
Application	Research use or in vitro diagnostics (IVD in EU-only)

Citations

2025 Lee et al., - Establishment of iPSC-Derived MSCs Expressing hsa-miR-4662a-5p for Enhanced Immune Modulation in Graft-Versus-Host Disease (GVHD), Int J Mol Sci, Species : Human ; Sample type : Cell culture supernatant ;
2025 Ueda et al., - Tryptophan-sensing receptor GPR142 expression levels are directly regulated by proinflammatory cytokines in ghrelin-producing cells, FEBS Open Bio. , Species : Human ; Sample type : Plasma ;
2025 Lim et al., - Single-cell transcriptomics of bronchoalveolar lavage during PRRSV infection with different virulence, Nat Commun., Species : Pig ; Sample type : Serum ;
2025 Fernández-Pinteño et al., - Age-Related Changes in Gut Health and Behavioral Biomarkers in a Beagle Dog Population, Animals, Species : Dog ; Sample type : Serum ;
2024 Dellink et al., - Continuous theta burst stimulation for bipolar depression: A multicenter, double-blind randomized controlled study exploring treatment efficacy and predictive potential of kynurenine metabolites, Journal of Affective Disorders, Species : Human ; Sample type : Plasma ;
2024 Valera et al., - Surface-Enhanced Raman Scattering Monitoring of Tryptophan Dynamics in 3D Pancreatic Tumor Models, ACS Sensors, Species : Human ; Sample type : Organoid ;
2024 Kim CE et al., - Induction of indoleamine 2,3-dioxygenase 1 expression in neurons of the central nervous system through inhibition of histone deacetylases blocks the progression of experimental autoimmune encephalomyelitis, Int Immunopharmacol., Species : Mouse ; Sample type : Serum ;
2024 Fongsaran C. et al., - VEEV TC-83 Triggers Dysregulation of the Tryptophan–Kynurenine Pathway in the Central Nervous System That Correlates with Cognitive Impairment in Tg2576 Mice, Pathogens, Species : Mouse ; Sample type : Brain ;
2024 Cecchi M. et al., - Unveiling the Role of Tryptophan 2,3-Dioxygenase in the Angiogenic Process, Pharmaceuticals, Species : Human ; Sample type : Cell culture supernatant ;
2024 Gillies et al., - Effects of a flavonoid-rich blackcurrant beverage on markers of the gut-brain axis in healthy females: Secondary findings from a 4-week randomized crossover control trial., Current Developments in Nutrition, Species : Human ; Sample type : Serum ;
2024 Vaughan et al., - Derangements of immunological proteins in HIV-associated diffuse large B-cell lymphoma: the frequency and prognostic impact, Front. Cell. Infect. Microbiol., Species : Human ; Sample type : Serum ;
2024 Jiménez-Trejo F. et al., - Downregulation of Serotonergic System Components in an Experimentally Induced Cryptorchidism in Rabbits, Int. J. Mol. Sci., Species : Rabbit ; Sample type : Testis ;
2024 Shu G. et al, - PABPC1L induces IDO1 to promote tryptophan metabolism and immune suppression in renal cell carcinoma, Cancer Res, Species : Human ; Sample type : Cell culture supernatant ;
2024 Wang Z. et al., - Camptothesome-based combination nanotherapeutic regimen for improved colorectal cancer immunochemotherapy, Biomaterials, Species : Mouse ; Sample type : Plasma ;
2024 R Gonzalez-Ponsand & JJ Bernard, - Benzo[a]pyrene exposure prevents high fat diet-induced obesity in the 4T1 model of mammary carcinoma, Front Oncol. , Species : Mice ; Sample type : Serum ;
2024 Ros-Lucas et al., - Transcriptomic evidence of immune modulation in subjects with chronic Trypanosoma cruzi infection, J Infect Dis., Species : Human ; Sample type : PBMC ;
2023 Dongen et al., - Atheroma plaque microenvironment stimulates kynurenine production by macrophages to induce endothelial adhesion molecules in the context of atherogenesis, bioRxiv, Species : Human ; Sample type : Plasma ;
2023 Mrstna et al., - Advances in kynurenine analysis, Clinica Chimica Acta, Species : Human ; Sample type : Plasma ;
2023 Wang et al., - Sphingomyelin-derived nanovesicles for the delivery of the IDO1 inhibitor epacadostat enhance metastatic and post-surgical melanoma immunotherapy, Nat Commun., Species : Mouse ; Sample type : Tumor tissues ;
2023 Letenneur et al., - Effects of a Lacticaseibacillus Mix on Behavioural, Biochemical, and Gut Microbial Outcomes of Male Mice following Chronic Restraint Stress, Nutrients, Species : Mouse ; Sample type : Serum ;
2023 Lysandrou et al., - Hypomethylation-induced regulatory programs in T cells unveiled by transcriptomic analyses, Frontiers in Immunology, Species : Human ; Sample type : Cell culture supernatant ;
2023 Munoz et al., - Effects of Supplementation with the Standardized Extract of Saffron (affron®) on the Kynurenine Pathway and Melatonin Synthesis in Rats, Antioxydants, Species : Rat ; Sample type : Serum ;
2023 Kaneko Y. et al, - Pathogenetic associations of anti-ribosomal P protein antibody titres and their subclasses in patients with systemic lupus erythematosus, Rheumatology (Oxford), Species : Human ; Sample type : Serum ;
2023 Onning et al., - Intake of Lactiplantibacillus plantarum HEAL9 Improves Cognition in Moderately Stressed Subjects: A Randomized Controlled Study, Nutrients, Species : Human ; Sample type : Plasma ;
2023 Mystakeli et al., - An open label randomized controlled trial of atorvastatin versus aspirin in elite controllers and antiretroviral-treated people with HIV, AIDS, Species : Human ; Sample type : Plasma ;
2023 Das et al., - Citrulline and kynurenine to tryptophan ratio: potential EED (environmental enteric dysfunction) biomarkers in acute watery diarrhea among children in Bangladesh, Scientific Reports, Species : Human ; Sample type : Plasma ;
2023 Cremone et al., - Unlocking the Secrets: Exploring the Biochemical Correlates of Suicidal Thoughts and Behaviors in Adults with Autism Spectrum Conditions, Biomedicines, Species : Human ; Sample type : Serum ;
2022 Struckmeier et al., - IDO1 is highly expressed in macrophages of patients in advanced tumour stages of oral squamous cell carcinoma, J of cancer research & clinical oncology, Species : Human ; Sample type : Blood ;
2022 Lischka et al., - Tryptophan Metabolism Is Associated with BMI and Adipose Tissue Mass and Linked to Metabolic Disease in Pediatric Obesity, Nutrients, Species : Human ; Sample type : Plasma ;
2021 Wood et al., - Apathy is not associated with a panel of biomarkers in older adults with HIV disease, J of Psychosomatic Research, Species : Human ; Sample type : Plasma ;
2021 Siska et al., - Kynurenine induces T cell fat catabolism and has limited suppressive effects in vivo, eBioMedicine, Species : Mouse ; Sample type : Tumor tissues ;
2021 Lee et al., - STING activation normalizes the intraperitoneal vascular-immune microenvironment and suppresses peritoneal carcinomatosis of colon cancer, J for ImmunoTherapy of Cancer, Species : Mouse ; Sample type : Tumor tissues ;
2021 Anzai et al., - IDO1-mediated Trp-kynurenine-AhR signal activation induces stemness and tumor dormancy in oral squamous cell carcinomas, Oral Science International, Species : Human ; Sample type : Cell culture supernatant ;
2021 Pospíšková et al., - Circulating concentrations of citrulline, neopterin, kynurenine, and tryptophan during chemoradiation in patients with cervical carcinoma, Pteridines, Species : Human ; Sample type : Plasma ;
2021 Kim et al., - Deep learning model enables the discovery of a novel immunotherapeutic agent regulating the kynurenine pathway, OncoImmunology, Species : Mouse ; Sample type : Tumor tissues ;
2021 Francikowski et al., - The effects of high-monosaccharide diets on development and biochemical composition of white-eyed mutant strain of house cricket (Acheta domesticus), Scientific reports, Species : Insects ; Sample type : Hemolymph ;
2021 Adu-Gyamfi et al., - Plasma Kynurenine-to-Tryptophan Ratio, a Highly Sensitive Blood-Based Diagnostic Tool for Tuberculosis in Pregnant Women Living With Human Immunodeficiency Virus (HIV), Clinical Infectious Diseases, Species : Human ; Sample type : Plasma ;
2021 Wang et al., - Abnormal Tryptophan Metabolism in HIV and Mycobacterium tuberculosis Infection, Front Microbiol., Species : Monkey ; Sample type : Plasma ;
2021 MacDowell et al., - Paliperidone Reversion of Maternal Immune Activation-Induced Changes on Brain Serotonin and Kynurenine Pathways, Frontiers in Pharmacology, Species : Mouse ; Sample type : Brain ;
2021 Itoh et al., - Cancer-associated fibroblasts educate normal fibroblasts to facilitate cancer cell spreading and T-cell suppression, Molecular Oncology, Species : Human ; Sample type : Cell culture supernatant ;
2020 Watanabe et al., - Adding Indoximod to Hypofractionated Radiotherapy with Anti-PD-1 Checkpoint Blockade Enhances Early NK and CD8+ T-Cell-Dependent Tumor Activity, Clinical Cancer Research, Species : Human ; Sample type : Cell culture supernatant ;
2020 Yeste et al., - Evaluating the potential role of tryptophan in calf milk replacers to facilitate weaning, Journal of Dairy Science, Species : Cattle ; Sample type : Plasma ;
2020 Nimii et al., - Effect of repetitive transcranial magnetic stimulation on the kynurenine pathway in stroke patients, Neuroreport. , Species : Human ; Sample type : Serum ;
2020 Baumgartner et al., - Evidence that a deviation in the kynurenine pathway aggravates atherosclerotic disease in humans, Journal of Internal Medicine, Species : Human ; Sample type : Plasma ;
2020 Adu-Gyamfi et al., - Diagnostic accuracy of plasma kynurenine/tryptophan ratio, measured by enzyme-linked immunosorbent assay, for pulmonary tuberculosis, International Journal of Infectious Diseases, Species : Human ; Sample type : Plasma ;
2020 Bekki et al. , - Serum kynurenine levels are a novel biomarker to predict the prognosis of patients with hepatocellular carcinoma, PLoS One, Species : Human ; Sample type : Serum ;
2020 Nafia et al., - IDO Targeting in Sarcoma: Biological and Clinical Implications, Front. Immunol, Species : Human ; Sample type : Cell culture ;
2020 Darvekar et al., - Selective Killing of Activated T Cells by 5-Aminolevulinic Acid Mediated Photodynamic Effect: Potential Improvement of Extracorporeal Photopheresis, Cancers, Species : Human ; Sample type : Cell culture supernatant ;
2019 Klar et al., - A highly efficient modality to block the degradation of tryptophan for cancer immunotherapy: locked nucleic acid-modified antisense oligonucleotides to inhibit human indoleamine 2,3-dioxygenase 1/tryptophan 2,3-dioxygenase expression, Cancer Immunology, Immunotherapy, Species : Human ; Sample type : Cell culture supernatant ;
2019 Hong et al., - Physical exercise prevents mice from L-Kynurenine-induced depression-like behavior, Asian Journal of Psychiatry, Species : Mouse ; Sample type : Plasma ;
2019 Allison et al., - Exercise training impacts skeletal muscle gene expression related to the kynurenine pathway, American Journal of Physiology, Species : Human ; Sample type : Plasma ;
2019 Qi et al., - Lactobacillus maintains healthy gut mucosa by producing L-Ornithine, Nature Communications Biology, Species : Mouse ; Sample type : Cell culture supernatant ;
2019 Agullo-Ortuno et al., - Blood Predictive Biomarkers for Patients With Non-small-cell Lung Cancer Associated With Clinical Response to Nivolumab, Clinical Lung Cancer, Species : Human ; Sample type : Plasma ;
2018 Ziklo et al., - Dysbiosis of the Vaginal Microbiota and Higher Vaginal Kynurenine/Tryptophan Ratio Reveals an Association with Chlamydia trachomatis Genital Infections, Front. Cell. Infect. Microbiol, Species : Human ; Sample type : Cervical secretion ;
2018 Gautam et al., - In vivo inhibition of tryptophan catabolism reorganizes the tuberculoma and augments immune-mediated control of Mycobacterium tuberculosis, Proc Natl Acad Sci U S A, Species : Monkey ; Sample type : Lung ;
2018 Martiin-Hernandez et al., - Chronic Mild Stress Alters Kynurenine Pathways Changing the Glutamate Neurotransmission in Frontal Cortex of Rats, Molecular Neurobiology, Species : Rat ; Sample type : Brain ;
2018 Zhang et al., - Traumatic Injury Leads to Inflammation and Altered Tryptophan Metabolism in the Juvenile Rabbit Brain, Journal of Neurotrauma, Species : Rabbit ; Sample type : Brain ;
2017 Toulmonde et al., - Use of PD-1 Targeting, Macrophage Infiltration, and IDO Pathway Activation in Sarcomas: A Phase 2 Clinical Trial, JAMA Oncology, Species : Human ; Sample type : Plasma ;
2017 Lee et al., - Inhibition of acute lethal pulmonary inflammation by the IDO-AhR pathway, PNAS, Species : Mouse ; Sample type : Tumor tissues ;
2017 Maliniemi et al., - Biological and clinical significance of tryptophan-catabolizing enzymes in cutaneous T-cell lymphomas, OncoImmunology, Species : Human ; Sample type : Cell culture supernatant ;
2017 Escobar-Alvarez et al., - The peroxyl radical-induced oxidation of Escherichia coli FtsZ and its single tryptophan mutant (Y222W) modifies specific side-chains, generates protein cross-links and affects biological function, Free Radical Biology and Medicine , Species : Bacteria ; Sample type : Cell culture ;
2017 Perez Guzman et al., - The Functional Relationship Between The Gut Microbiota And Generalized Anxiety Disorder in The Murine Model, Thesis - McMaster University, Species : Mouse ; Sample type : Serum ;

Frequently asked questions

BA-E-2200

L-Kynurenine ELISA kit

The L-Kynurenine ELISA kit is a user-friendly and highly sensitive assay designed for reliable quantification of kynurenine in a wide range of biological samples. Optimized for use with plasma, serum, and cell culture supernatants, this assay requires only 20 µL of sample and offers a low detection limit of 45.7 ng/mL—ideal for longitudinal studies, even in small animal models such as mice.

Validated across multiple species, the KYN ELISA has been successfully used in peer-reviewed studies to measure kynurenine levels in human, monkey, dog, pig, mouse, rat, rabbit, cattle, and insect samples. Whether you’re conducting basic research, preclinical studies, or clinical investigations, this versatile kit ensures accurate and reproducible results across large sample sets.

€599

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For questions, protocol recommendations, and troubleshooting, you have direct access to the scientist that developed the antibody or ELISA kit you’re using.

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A knowledge base containing protocols, Frequently Asked Questions, and troubleshooting tips is also available online.

They published with this product

Johns Hopkins University School f Medicine

First Affiliated Hospital of Sun Yat-sen University

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Antibodies

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Antibodies

L-Kynurenine Antibody – Rabbit Polyclonal

Used for IHC in FFPE lung & tumor tissues. Cited in literature

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L-tryptophan ELISA kit high sensitive any sample

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Amino acids

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BA-E-2200

L-Kynurenine ELISA kit

Kynurenine ELISA kit – Research test for the detection of L-Kynurenine in biological fluids and cell culture supernatant

Specifications

Product name	L-Kynurenine ELISA kit
Description	Enzyme immunoassay (ELISA) for the quantitative determination of L-Kynurenine in biological fluids and cell supernatant.
Format	96-well plate
Samples	Plasma, serum, cell culture supernatant
Minimal sample volume	20µL
Reactivity	Reacts with all species
Standard range	100 – 10,000ng/mL
Sensitivity	LoD: 47.5ng/mL
Specificity	No significant cross-reactivity was observed with L-Kynurenine analogs such as L-Tryptophan, 3-Hydroxy-DL-Kynurenine, 5-Hydroxy-DL-Tryptophan, Tyrosine, Serotonin, Phenylalanin, L-Asparagine and Kynurenic acid
Assay time	Sample acetylation (90-120 min), overnight incubation, ELISA revelation & read step (1h)

Instructions

Storage	Store at 2-8°C for to 6 months
Sample collection & storage	Plasma/Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands. Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months)
Sample preparation	Sample acylation (90 min)
ELISA	Antisera overnight incubation, revelation and read steps (1h)