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L-tryptophan ELISA kit high sensitive any sample

Tryptophan ELISAStandard curve - to be generated every time the assay is performed.

Determination of L-Tryptophan levels in CT26 cell cultures by ELISAMurine CT26 tumor cell line was stably transfected with either empty vector (Mock) or vector containing cDNA encoding for indoleamine 2,3 dioxygenase (IDO1). Cells were cultured for 48 hours and Tryptophan was measured using the Tryptophan ELISA kit. As expected, Tryptophan level was significantly lower in IDO1-proficient CT26 cells than in mock or parental (WT) CT26 cell lines.

Key features

Sample type	Biological fluids (urine, plasma, serum, …), cell culture supernatant
Capacity	96 tests
Sensitivity	LoD: < 1.2µg/mL
Range	2.5 – 250µg/mL
Assay time	Sample prep (2h30), overnight incubation, ELISA revelation & read step (1h)
Reactivity	Reacts with all species
Application	Research use & in vitro diagnostics (IVD label in EU only)

Citations

2025 Ueda et al., - Tryptophan-sensing receptor GPR142 expression levels are directly regulated by proinflammatory cytokines in ghrelin-producing cells, FEBS Open Bio. , Species : Human ; Sample type : Plasma ;
2025 Lim et al., - Single-cell transcriptomics of bronchoalveolar lavage during PRRSV infection with different virulence, Nat Commun., Species : Pig ; Sample type : Serum ;
2025 Fernández-Pinteño et al., - Age-Related Changes in Gut Health and Behavioral Biomarkers in a Beagle Dog Population, Animals, Species : Dog ; Sample type : Serum ;
2024 Dellink et al., - Continuous theta burst stimulation for bipolar depression: A multicenter, double-blind randomized controlled study exploring treatment efficacy and predictive potential of kynurenine metabolites, Journal of Affective Disorders, Species : Human ; Sample type : Plasma ;
2024 Valera et al., - Surface-Enhanced Raman Scattering Monitoring of Tryptophan Dynamics in 3D Pancreatic Tumor Models, ACS Sensors, Species : Human ; Sample type : Organoid ;
2024 Fongsaran C. et al., - VEEV TC-83 Triggers Dysregulation of the Tryptophan–Kynurenine Pathway in the Central Nervous System That Correlates with Cognitive Impairment in Tg2576 Mice, Pathogens, Species : Mouse ; Sample type : Brain ;
2024 Gillies et al., - Effects of a flavonoid-rich blackcurrant beverage on markers of the gut-brain axis in healthy females: Secondary findings from a 4-week randomized crossover control trial., Current Developments in Nutrition, Species : Human ; Sample type : Serum ;
2024 Vaughan et al., - Derangements of immunological proteins in HIV-associated diffuse large B-cell lymphoma: the frequency and prognostic impact, Front. Cell. Infect. Microbiol., Species : Human ; Sample type : Serum ;
2024 Shu G. et al, - PABPC1L induces IDO1 to promote tryptophan metabolism and immune suppression in renal cell carcinoma, Cancer Res, Species : Human ; Sample type : Cell culture supernatant ;
2024 Wang Z. et al., - Camptothesome-based combination nanotherapeutic regimen for improved colorectal cancer immunochemotherapy, Biomaterials, Species : Mouse ; Sample type : Plasma ;
2024 Ros-Lucas et al., - Transcriptomic evidence of immune modulation in subjects with chronic Trypanosoma cruzi infection, J Infect Dis., Species : Human ; Sample type : PBMC ;
2023 Letenneur et al., - Effects of a Lacticaseibacillus Mix on Behavioural, Biochemical, and Gut Microbial Outcomes of Male Mice following Chronic Restraint Stress, Nutrients, Species : Mouse ; Sample type : Serum ;
2023 Onning et al., - Intake of Lactiplantibacillus plantarum HEAL9 Improves Cognition in Moderately Stressed Subjects: A Randomized Controlled Study, Nutrients, Species : Human ; Sample type : Plasma ;
2023 Wang et al., - Sphingomyelin-derived nanovesicles for the delivery of the IDO1 inhibitor epacadostat enhance metastatic and post-surgical melanoma immunotherapy, Nat Commun., Species : Mouse ; Sample type : Tumor tissues ;
2023 Lysandrou et al., - Hypomethylation-induced regulatory programs in T cells unveiled by transcriptomic analyses, Frontiers in Immunology, Species : Human ; Sample type : Cell culture supernatant ;
2023 Munoz et al., - Effects of Supplementation with the Standardized Extract of Saffron (affron®) on the Kynurenine Pathway and Melatonin Synthesis in Rats, Antioxydants, Species : Rat ; Sample type : Serum ;
2023 Kaneko Y. et al, - Pathogenetic associations of anti-ribosomal P protein antibody titres and their subclasses in patients with systemic lupus erythematosus, Rheumatology (Oxford), Species : Human ; Sample type : Serum ;
2023 Mystakeli et al., - An open label randomized controlled trial of atorvastatin versus aspirin in elite controllers and antiretroviral-treated people with HIV, AIDS, Species : Human ; Sample type : Plasma ;
2023 Das et al., - Citrulline and kynurenine to tryptophan ratio: potential EED (environmental enteric dysfunction) biomarkers in acute watery diarrhea among children in Bangladesh, Scientific Reports, Species : Human ; Sample type : Plasma ;
2023 Mrstna et al., - Advances in kynurenine analysis, Clinica Chimica Acta, Species : Human ; Sample type : Plasma ;
2023 Dongen et al., - Atheroma plaque microenvironment stimulates kynurenine production by macrophages to induce endothelial adhesion molecules in the context of atherogenesis, bioRxiv, Species : Human ; Sample type : Plasma ;
2022 Struckmeier et al., - IDO1 is highly expressed in macrophages of patients in advanced tumour stages of oral squamous cell carcinoma, J of cancer research & clinical oncology, Species : Human ; Sample type : Blood ;
2022 Lischka et al., - Tryptophan Metabolism Is Associated with BMI and Adipose Tissue Mass and Linked to Metabolic Disease in Pediatric Obesity, Nutrients, Species : Human ; Sample type : Plasma ;
2021 Wood et al., - Apathy is not associated with a panel of biomarkers in older adults with HIV disease, J of Psychosomatic Research, Species : Human ; Sample type : Plasma ;
2021 Park et al., - Early-Life Stress Modulates Gut Microbiota and Peripheral and Central Inflammation in a Sex-Dependent Manner, International j of molecular sciences, Species : Rat ; Sample type : Brain ;
2021 Pospíšková et al., - Circulating concentrations of citrulline, neopterin, kynurenine, and tryptophan during chemoradiation in patients with cervical carcinoma, Pteridines, Species : Human ; Sample type : Plasma ;
2021 Kim et al., - Deep learning model enables the discovery of a novel immunotherapeutic agent regulating the kynurenine pathway, OncoImmunology, Species : Mouse ; Sample type : Tumor tissues ;
2021 Francikowski et al., - The effects of high-monosaccharide diets on development and biochemical composition of white-eyed mutant strain of house cricket (Acheta domesticus), Scientific reports, Species : Insects ; Sample type : Hemolymph ;
2021 Adu-Gyamfi et al., - Plasma Kynurenine-to-Tryptophan Ratio, a Highly Sensitive Blood-Based Diagnostic Tool for Tuberculosis in Pregnant Women Living With Human Immunodeficiency Virus (HIV), Clinical Infectious Diseases, Species : Human ; Sample type : Plasma ;
2021 Wang et al., - Abnormal Tryptophan Metabolism in HIV and Mycobacterium tuberculosis Infection, Front Microbiol., Species : Monkey ; Sample type : Plasma ;
2021 MacDowell et al., - Paliperidone Reversion of Maternal Immune Activation-Induced Changes on Brain Serotonin and Kynurenine Pathways, Frontiers in Pharmacology, Species : Mouse ; Sample type : Brain ;
2020 Watanabe et al., - Adding Indoximod to Hypofractionated Radiotherapy with Anti-PD-1 Checkpoint Blockade Enhances Early NK and CD8+ T-Cell-Dependent Tumor Activity, Clinical Cancer Research, Species : Human ; Sample type : Cell culture supernatant ;
2020 Nimii et al., - Effect of repetitive transcranial magnetic stimulation on the kynurenine pathway in stroke patients, Neuroreport. , Species : Human ; Sample type : Serum ;
2020 Baumgartner et al., - Evidence that a deviation in the kynurenine pathway aggravates atherosclerotic disease in humans, Journal of Internal Medicine, Species : Human ; Sample type : Plasma ;
2020 Adu-Gyamfi et al., - Diagnostic accuracy of plasma kynurenine/tryptophan ratio, measured by enzyme-linked immunosorbent assay, for pulmonary tuberculosis, International Journal of Infectious Diseases, Species : Human ; Sample type : Plasma ;
2020 Nafia et al., - IDO Targeting in Sarcoma: Biological and Clinical Implications, Front. Immunol, Species : Human ; Sample type : Cell culture ;
2019 Agullo-Ortuno et al., - Blood Predictive Biomarkers for Patients With Non-small-cell Lung Cancer Associated With Clinical Response to Nivolumab, Clinical Lung Cancer, Species : Human ; Sample type : Plasma ;
2018 Ziklo et al., - Dysbiosis of the Vaginal Microbiota and Higher Vaginal Kynurenine/Tryptophan Ratio Reveals an Association with Chlamydia trachomatis Genital Infections, Front. Cell. Infect. Microbiol, Species : Human ; Sample type : Cervical secretion ;
2018 Gautam et al., - In vivo inhibition of tryptophan catabolism reorganizes the tuberculoma and augments immune-mediated control of Mycobacterium tuberculosis, Proc Natl Acad Sci U S A, Species : Monkey ; Sample type : Lung ;
2018 Martiin-Hernandez et al., - Chronic Mild Stress Alters Kynurenine Pathways Changing the Glutamate Neurotransmission in Frontal Cortex of Rats, Molecular Neurobiology, Species : Rat ; Sample type : Brain ;
2018 Zhang et al., - Traumatic Injury Leads to Inflammation and Altered Tryptophan Metabolism in the Juvenile Rabbit Brain, Journal of Neurotrauma, Species : Rabbit ; Sample type : Brain ;
2017 Toulmonde et al., - Use of PD-1 Targeting, Macrophage Infiltration, and IDO Pathway Activation in Sarcomas: A Phase 2 Clinical Trial, JAMA Oncology, Species : Human ; Sample type : Plasma ;
2017 Perez Guzman et al., - The Functional Relationship Between The Gut Microbiota And Generalized Anxiety Disorder in The Murine Model, Thesis - McMaster University, Species : Mouse ; Sample type : Serum ;
2016 Ziklo et al., - In vitro rescue of genital strains of Chlamydia trachomatis from interferon-γ and tryptophan depletion with indole-positive, but not indole-negative Prevotella spp, ., Species : Bacteria ; Sample type : Cell culture supernatant ;

Frequently asked questions

BA-E-2700

L-Tryptophan ELISA kit

The L-Tryptophan ELISA kit offers a robust and sensitive method for the quantitative measurement of L-Tryptophan in biological fluids and cell culture supernatants. Designed for both in vitro diagnostic and research applications, the assay is formatted on a 96-well plate — ideal for the rapid processing of large sample cohorts.

This assay enables precise quantification from minimal sample volumes (as low as 20 µL) and has been cross-validated by LC-MS, ensuring excellent analytical performance. Applicable to all species, the kit has been successfully used in peer-reviewed studies involving human, monkey, dog, pig, mouse, rat, rabbit, and insect samples. Whether in basic research, preclinical development, or clinical investigations, this ELISA provides a reliable tool for studying tryptophan metabolism.

€599

Number of citations :

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For questions, protocol recommendations, and troubleshooting, you have direct access to the scientist that developed the antibody or ELISA kit you’re using.

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A knowledge base containing protocols, Frequently Asked Questions, and troubleshooting tips is also available online.

They published with this product

Johns Hopkins University School f Medicine

The University of Texas MD Anderson Cancer Center

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BA-E-2700

L-Tryptophan ELISA kit

Tryptophan ELISA kit – Research test for the detection of L-Tryptophan

Specifications

Product name	Tryptophan ELISA kit
Description	Enzyme immunoassay (ELISA) for the quantitative determination of L-Tryptophan in biological fluids (urine, plasma, serum samples, …) and cell culture supernatant
Format	96-well plate
Samples	Urine, plasma, serum, urine, cell culture supernatant
Minimal sample volume	20µL
Reactivity	Reacts with all species
Standard range	2.5 – 250µg/mL
Sensitivity	1.2µg/mL
Specificity	No significant cross-reactivity was observed with L-Tryptophan analogs such as 5-Hydroxy-L-tryptophan, Tryptamine, 5-Methoxytryptophan, 5-Hydroxytryptamine, N-acetyl-5-hydroxytryptamine
Assay time	Sample preparation (2h30), overnight incubation, ELISA revelation & read step (1h)

Instructions

Storage	Store at 2-8°C for to 6 months
Sample collection & storage	Urine: spontaneous or 24-hour urine collected in bottle with 10-15 mL of 6 M HCl, stored at -20°C for long periods (up to 6 months) Plasma/Serum: EDTA-, Heparin- or Citrate- samples stored at 2-8°C for up to 48h or -20°C for longer period (up to 6 months). Do not use haemolytic or lipemic samples
Sample preparation	Sample precipitation (15 min) and derivatization (2h15)
ELISA	Antisera overnight incubation, revelation and read steps (1h)