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Tryptophan detection in the crayfish brainAnti-Tryptophan antibody demonstrates the presence of Tryptophan positive cells in the brain of a crayfish. Staining was obtained with mouse monoclonal anti-Tryptophan antibody (IS011) and performed with STAINperfect immunostaining kit A, following the protocol for whole mount samples. Fluorescent conjugated secondary antibody was used and images obtained by confocal imaging at high magnification (x40).
Tryptophan immunostaining in the CNS of embryonic mouseUsing STAINperfect immunostaining kit A, Tryptophan was detected in the hindbrain of embryonic mouse E15.5. Staining was obtained following the protocol optimized for whole mount using IS011 mouse monoclonal antibody targeting L-Tryptophan. Fluorescent conjugated secondary antibodies were then used and images captured by confocal microscopy at high magnification.
Affinity & specificity of anti- L-Tryptophan antibodyCompetitive ELISA demonstrates that low amounts of L-Tryptophan conjugate are required to abolish antigen-antibody reaction (high affinity), while rising concentrations of L-Tryptophan analog tryptamine do not affect reaction (high specificity).
Amino acid L-TryptophanL-Tryptophan is an essential amino acid, which is converted into Serotonin by tryptophan hydroxylase (TPH) in the gut or brain, or catabolized by various cell types into L-Kynurenine through indoleamine 2,3-dioxygenase (IDO1/2) or tryptophan 2,3-dioxygenase (TDO2) . Abnormal tryptophan metabolism is implicated in a wide range of pathological states, including metabolic and auto-immune diseases, cancer, viral infections, as well as neurodegenerative and mood disorders.
Key features
| Clonality | Monoclonal antibody |
| Host | Mouse |
| Reactivity | Reacts with all species |
| Tested samples | Human cell cultures, rodent brain and renal tissues |
| Staining procedure | STAINperfect immunostaining kit A (protocols for cell cultures, whole mounts, tissue sections) |
| Format | 50µL (approx. 40 tissue sections) |
Frequently asked questions
L-Tryptophan Antibody – Mouse Monoclonal
The anti-tryptophan antibody IS011 was validated in mouse and crayfish brain tissues using the STAINperfect Immunostaining Kit A. Additionally, this monoclonal antibody has been successfully used in recent scientific publications to stain human cells and mouse kidney tissue sections.
€449
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For questions, protocol recommendations, and troubleshooting, you have direct access to the scientist that developed the antibody or ELISA kit you’re using.
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IS011
L-Tryptophan Antibody – Mouse Monoclonal
Anti-L-Tryptophan mAb – Purified monoclonal antibody for research use only (Liquid 50µL)
Specifications
| Product name | L-Tryptophan antibody |
| Synonyms | Anti-L-Trp antibody
(S)-2-Amino-3-(3-indolyl)propionic acid antibody L-alpha-Amino-3-indolepropionic acid antibody (S)-2-Amino-3-(3-indolyl)propanoic acid antibody L-alpha-Amino-3-indolepropanoic acid antibody |
| Immunogen | Conjugated L-Tryptophan |
| Isotype |
IgG1 k chain |
| Clone |
Clone 4H11-A11 |
| Specificity | When tested in competitive ELISA, the anti-conjugated L-Tryptophan antibody did not show any significant cross reactivity with Tryptophan analogs, including Tryptamine |
| Volume | 50 µL |
| Form | Liquid |
| Purity | Purified IgG |
Instructions
| Storage |
Store at +4°C for short term (6 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Immunofluorescence (IF) |
Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample (Cell cultures, Whole mounts, Tissue sections) |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
