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Key features
| Sample type | Plasma or serum |
| Capacity | 96-well plate |
| Sensitivity | 1,6 pg/mL |
| Range | 0/3 – 300 pg/mL |
| Assay time | Sample extraction (15 min), incubation (overnight), ELISA (3h) |
| Reactivity | Reacts with all species |
| Application | Research use |
| Material required | Evaporator centrifuge (Speed-Vac) or sample concentrator by use of nitrogen (e.g. Techne) |
Frequently asked questions
Melatonin ELISA kit
The Melatonin ELISA kit allows for the easy and accurate quantification of N-acetyl-5-methoxy-tryptamine (melatonin) in plasma and serum samples. With a detection limit of 1.6 pg/mL, the kit enables the measurement of melatonin in blood during both daytime (0 to 20 pg/mL) and nighttime (40 to 100 pg/mL).
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For questions, protocol recommendations, and troubleshooting, you have direct access to the scientist that developed the antibody or ELISA kit you’re using.
Direct customer support is available all business days.
A knowledge base containing protocols, Frequently Asked Questions, and troubleshooting tips is also available online.
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Melatonin ELISA kit
Melatonin ELISA kit – Research test for the detection of Melatonin
Specifications
| Product name | Melatonin ELISA kit – Highly Sensitive – Plasma & Serum Samples |
| Description | Research enzyme immunoassay (ELISA) for the sensitive determination of Melatonin levels in plasma and serum samples |
| Format | 96-well plate |
| Samples | Plasma, serum |
| Minimal sample volume | 500 µL |
| Reactivity | Reacts with all species |
| Standard range | 0 / 3 – 300 pg/mL |
| Sensitivity |
1,6 pg/mL |
| Specificity | No significant cross-reactivity was observed with Melatonin analogs such as 5-Methoxy-Tryptophole, N-Acetyl-Serotonin, 5-Methoxy-Tryptamine |
| Assay time | Sample extraction (15 min), incubation (overnight), ELISA (3h) |
Instructions
| Storage | Store at 2-8°C for to 6 months |
| Sample collection & storage | EDTA or Heparin plasma or serum samples only. Do not use haemolytic, icteric or lipemic samples. Storage: up to 24 hours at 2 – 8°C; for longer periods (up to 3 months) at – 20°C |
| Sample preparation | Sample preparation, extraction and acylation (15min) |
| ELISA | Melatonin antiserum incubation (overnight), revelation and read steps (3h) |
