The Phosphoethanolamine ELISA Kit (IS I-3500R) is designed for the quantitative measurement of phosphoethanolamine in human plasma samples. It can support research in metabolism, biochemistry, oncology, and biomarker discovery.

This kit is based on the competitive ELISA principle. Plasma samples undergo extraction and derivatization, and phosphoethanolamine in the processed sample competes with the antigen coated on the microplate for antibody binding sites. The bound antibodies are detected using an HRP-conjugated secondary antibody and TMB substrate. The absorbance measured at 450 nm is inversely proportional to the phosphoethanolamine concentration.

The assay has been validated for EDTA plasma samples. Hemolytic or lipemic samples should be avoided because they may interfere with accuracy. Other sample types, such as serum, may be used at the responsibility of the user, but should be validated individually.

The Phosphoethanolamine ELISA Kit has a quantitative measuring range of 50.6 to 1128.4 ng/mL, with a limit of detection of 24 ng/mL.

Whole blood should be collected in EDTA tubes and centrifuged immediately at room temperature. Plasma can be stored for up to 48 hours at 2–8 °C. For longer periods, samples should be frozen at –20 °C for up to 6 months. Repeated freeze–thaw cycles should be avoided.

Each kit contains reagents sufficient for 96 determinations, including standards and controls. Depending on the number of replicates, this typically allows testing of approximately 40 to 44 unknown samples in duplicate.

The kit includes precoated 96-well microplate strips, standards covering a range from 0 to 1128 ng/mL, quality controls, phosphoethanolamine antiserum, enzyme conjugate, substrate solution, stop solution, wash buffer, equalizing, precipitation, and derivatization reagents, as well as adhesive foils and assay buffers. A detailed list of components is provided in the Instructions for Use.

The assay is highly specific for phosphoethanolamine. Cross-reactivity testing shows less than 0.1 percent reactivity with phosphoserine or 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine.

The Phosphoethanolamine ELISA demonstrates intra-assay coefficients of variation between 3.5 and 7.4 percent. Recovery experiments show values between 93 and 101 percent, and dilution linearity up to 1:64 is maintained within 79 to 101 percent.

Running the assay requires a microplate reader capable of reading at 450 nm with a reference wavelength between 620 and 650 nm, a microplate washer, precision pipettes, a vortex mixer, and a microplate shaker operating at approximately 600 rpm.

No. The Phosphoethanolamine ELISA Kit is for research use only. It must not be used for diagnostic or therapeutic applications.

Phosphoethanolamine is a central metabolite in phospholipid metabolism and is increasingly studied as a biomarker in cancer, metabolic disorders, and neurological diseases. This ELISA kit offers a robust and convenient method for investigating its biological and clinical relevance.