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Immunohistochemistry against serotonin (5HT) on a FFPE human colon coronal section.5HT immunoreactivity, revealed using anti-serotonin (5HT, rabbit polyclonal antibody #IS1086), is highlighted in enterochromaffin cells of the intestinal mucosa.
Immunohistochemistry against serotonin (5HT) on a FFPE human colon coronal section5HT immunoreactivity, revealed using anti-serotonin (5HT, rabbit polyclonal antibody #IS1086), is highlighted in enterochromaffin cells of the intestinal mucosa.
Serotonergic neurons of the Raphe nucleus through a FFPE mouse brainstem coronal sectionrevealed by anti-serotonin (5HT, rabbit polyclonal antibody #IS1086) and Microtubule-Associated Protein-2 (MAP2) immunohistofluorescence. 5HT immunoreactivity highlights well the 5HT-containing neurons of the dorsal (DRN).
Affinity & specificity of anti-Serotonin rabbit polyclonal antibodyCompetitive ELISA highlights that low amounts of conjugated Serotonin are required to abolish antigen-antibody reaction (high affinity), while rising concentrations of analog conjugates (5-Hydroxy-Tryptophan, Tryptamine or Tryptophan) affect only to a lesser extent the reaction (high specificity).
Key features
| Clonality | Polyclonal antibody |
| Host | Rabbit |
| Reactivity | Reacts with all species |
| Tested samples | Human tumor samples, Mouse brain tissues |
| Staining procedure | IHC / IHF |
| Format | 50µL (approx. 40 tissue sections) |
Frequently asked questions
What is the Serotonin antibody for FFPE samples IS1086 used for?
This antibody is designed for the specific detection of serotonin (5-hydroxytryptamine, 5-HT) in formalin-fixed paraffin-embedded (FFPE) tissues, making it suitable for studies of the serotonergic system in various biological models.
Which applications are validated for this antibody?
The Serotonin antibody for FFPE has been validated for immunohistochemistry (IHC) and immunofluorescence (IF) on human and mouse FFPE samples.
From which species can this antibody detect serotonin?
The antibody is applicable to any species. It has been successfully tested on human and mouse FFPE samples.
What type of antibody is it?
It is a rabbit polyclonal antibody (catalog #IS1086) raised against serotonin (5-HT).
Is this antibody compatible with formalin-fixed tissues?
Yes. This Serotonin antibody is specifically designed for use on formalin-fixed biological materials, including paraffin-embedded sections.
What are the main advantages of using this antibody?
- High specificity for serotonin (5-HT) - Robust performance in FFPE samples - Validated in IHC and IF applications - Suitable across multiple species
Serotonin Antibody for FFPE samples – Rabbit Polyclonal
Our Serotonin antibody for FFPE samples (#IS1086, rabbit polyclonal) is specifically designed for reliable detection of serotonin (5-hydroxytryptamine, 5-HT) in formalin-fixed paraffin-embedded tissues. This antibody enables accurate visualization of the serotonergic system and has been thoroughly validated for both immunohistochemistry (IHC) and immunofluorescence (IF) applications in mouse and human specimens.
€ 449
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IS1086
Serotonin Antibody for FFPE samples – Rabbit Polyclonal
Anti-Serotonin pAb for FFPE samples – Purified polyclonal antibody for research use only (liquid 50µL)
Specifications
| Product name | Serotonin antibody – 5-HT rabbit polyclonal Ab |
| Synonyms | Anti-5-HT antibody Anti-5-hydroxytryptamine antibody |
| Immunogen | Conjugated 5-HT |
| Specificity | When tested in competitive ELISA, the anti-conjugated serotonin antibody did not cross-react with 5-hydroxytryptamine analogs, including tryptamine and 5-hydroxytryptophan |
| Volume | 50 µL |
| Form | Liquid |
| Purity | Purified anti-serum |
Instructions
| Storage |
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| IHC/IF |
Dilute at 1/250 -1/1000 |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
