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5-HT -antibody Immunolabeling of Octopaminergic and Serotoninergic neuronsImmunostaining of crayfish eyetalk using anti-octopamine rabbit polyclonal antibody (green) and anti-serotonin goat polyclonal antibody (red). Tissus were processed with whole mount protocol of STAINperfect immunostaining kit A. Fluorescent labeled secondary antibodies were used and pictures were acquired by confocal imaging with high magnification.
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Affinity & specificity of anti-Serotonin antibodyCompetitive ELISA demonstrates that low amounts of Serotonin conjugate are required to abolish antigen-antibody reaction (high affinity), while rising concentrations of Serotonin competitors Tryptamine or 5-MethoxyTryptamine do not affect reaction (high specificity).
Key features
| Clonality | Polyclonal antibody |
| Host | Rabbit |
| Reactivity | Reacts with all species |
| Tested samples | Mouse primary neurons and brain tissues, crayfish tissues |
| Staining procedure | STAINperfect immunostaining kit A (protocols for cell cultures, whole mounts, tissue sections) |
| Format | 50µL (approx. 40 tissue sections) |
Frequently asked questions
IS1035
Serotonin Antibody – Goat Polyclonal
The rabbit polyclonal anti-serotonin antibody IS1035 was validated using the STAINperfect immunostaining kit A in mouse primary neurons, mouse brain tissues, and crayfish brain tissues, demonstrating robust and specific labeling of serotonin (5-HT).
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IS1035
Serotonin Antibody – Goat Polyclonal
Anti-Serotonin pAb – Purified polyclonal antibody for research use only (liquid 50µL)
Specifications
| Product name | Serotonin antibody (5-HT goat polyclonal Ab) |
| Synonyms | Anti-5-HT antibodyAnti-5-hydroxytryptamine antibody Anti-hydroxytryptamine antibody |
| Immunogen | Conjugated 5-HT |
| Specificity | When tested in competitive ELISA, the anti-conjugated Serotonin antibody did not show any significant cross reactivity with 5-Hydroxytryptamine analogs, including Tryptamine and 5-HydroxyTryptophan |
| Volume | 50 µL |
| Form | Liquid |
| Purity | Purified anti-serum |
Instructions
| Storage |
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Immunofluorescence (IF) |
Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample (Cell cultures, Whole mounts, Tissue sections) |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
