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Serotonin / Tyrosine Hydroxylase in mouse embryonic midbrainImmunodetection of serotonin (red – IS040) and Tyrosine hydroxylase (green) in the mouse embryonic midbrain (E13.5) using mouse monoclonal anti-serotonin antibody (IS040) and rabbit polyclonal anti-TH antibody
Serotonin neurons and fibers in embryonic mouse medullaUsing STAINperfect immunostaining kit A, the anti-Serotonin mouse monoclonal antibody (Ref: IS040) demonstrates the presence of serotonin in embryonic mouse medulla (E13.5). The staining was performed according to the protocol optimized for whole-mount samples and was revealed using fluorescent conjugated secondary antibody.
Affinity & specificity of anti-Serotonin antibodyCompetitive ELISA demonstrates that low amounts of Serotonin conjugate are required to abolish antigen-antibody reaction (high affinity), while rising concentrations of Serotonin competitors 5-Methoxy-Tryptamine or Tryptamine do not affect reaction (high specificity).
Serotonin – 5-hydroxytryptamine (5-HT)Serotonin is a monoamine neurotransmitter derived from tryptophan metabolism. Either produced by gut cells or synthesized by serotonergic neurons in the brain, Serotonin is known to be involved in the regulation of appetite, mood, sleep, memory, aging, and to modulate both innate and adaptive immune responses.
Key features
| Clonality | Monoclonal antibody |
| Host | Mouse |
| Reactivity | Reacts with all species |
| Tested samples | Mouse brain tissues, Human brain organoids |
| Staining procedure | STAINperfect immunostaining kit A (protocols for cell cultures, whole mounts, tissue sections) |
| Format | 50µL (approx. 40 tissue sections) |
Frequently asked questions
Serotonin Antibody – Mouse Monoclonal
Anti-Serotonin antibody IS040, in combination with the STAINperfect immunostaining kit A, enables reliable detection of 5-HT in whole mounts, cell cultures, and tissue sections. Our team successfully used this antibody to stain embryonic mouse tissues, and recent studies have demonstrated its effectiveness in visualizing serotonin in human midbrain organoids and mouse brain tissues.
€449
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IS040
Serotonin Antibody – Mouse Monoclonal
Anti-Serotonin mAb – Purified monoclonal antibody for research use only (liquid 50µL)
Specifications
| Product name | Serotonin antibody – mouse mAb |
| Synonyms | Anti-5-HT antibody
Anti-5-hydroxytryptamine antibody |
| Immunogen | Conjugated 5-HT |
| Isotype |
IgG1 k chain |
| Clone |
Clone 2E1F6 |
| Specificity | When tested in competitive ELISA, the anti-conjugated Serotonin antibody did not show any significant cross reactivity with 5-Hydroxytryptamine analogs, including Tryptamine and 5-Methoxytryptamine |
| Volume | 50 µL |
| Form | Liquid |
| Purity | Purified IgG |
Instructions
| Storage |
Store at +4°C for short term (6 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Immunofluorescence (IF) |
Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample (Cell cultures, Whole mounts, Tissue sections) |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
