Related products
D-Serine Antibody – Rabbit Polyclonal
€449
Dopamine Antibody – Rabbit Polyclonal
€449
L-Glutamate Antibody – Rabbit Polyclonal
€449
L-Serine Antibody – Rabbit Polyclonal
€449
GABA Antibody – Rabbit Polyclonal
€449
Anti-Serotonin antibody Staining of serotoninergic neurons in mouse primary hindbrain cultureAnti-Serotonin (green) and MAP2 (red) antibodies permit the detection of serotoninergic neurons in primary mouse hindbrain cell culture. Staining was performed using STAINperfect immunostaining kit A, according to the protocol optimized for cell culture and compatible with a MAP2 staining. Fluorescent labeled secondary antibody were used and picture was acquired by high-content imaging.
Presence of serotonin in neurons of crayfish brainHigh magnification of serotoninergic neurons in the brain of a crayfish. This staining shows serotonin presence in soma containing vesicules and projections. Tissue was processed following whole mount protocol of STAINperfect immunostaining kit A. Fluorescent labeled secondary antibody was used and picture was acquired by confocal imaging.
Serotonin imaging in embryonic mouse medullaUsing STAINperfect immunostaining kit A, the anti-Serotonin rabbit polyclonal antibody (Ref: IS1007) demonstrates the presence of serotonin in embryonic mouse medulla (E13.5). The staining was performed according to the protocol optimized for whole-mount samples and was revealed using Alexa Fluor® 488-conjugated secondary antibody.
Immunostaining of 5-HT-containing cells in adult mouse brain sliceAnti-Serotonin antibody highlights the soma of serotoninergic neurons in the pons of mouse adult brain sagittal section. Staining was performed with STAINperfect immunostaining kit A, following the protocol for tissue sections. Secondary antibody (goat anti-rabbit Alexa Fluor® 488) was used and picture was acquired by confocal imaging at high magnification.
Affinity & specificity of anti-5-HT antibodyCompetitive ELISA demonstrates that low amounts of Serotonin conjugate are required to abolish antigen-antibody reaction (high affinity), while rising concentrations of Tryptamine and 5-Methoxy-Tryptamine conjugates do not affect reaction (high specificity).
Serotonin – 5-hydroxytryptamine (5-HT)Serotonin is a monoamine neurotransmitter derived from tryptophan metabolism. Either produced by gut cells or synthesized by serotonergic neurons in the brain, Serotonin is known to be involved in the regulation of appetite, mood, sleep, memory, aging, and to modulate both innate and adaptive immune responses.
Key features
| Clonality | Polyclonal antibody |
| Host | Rabbit |
| Reactivity | Reacts with all species |
| Tested samples | Mouse primary neurons and brain tissues, crayfish tissues |
| Staining procedure | STAINperfect immunostaining kit A (protocols for cell cultures, whole mounts, tissue sections) |
| Format | 50µL (approx. 40 tissue sections) |
Frequently asked questions
Serotonin Antibody – Rabbit Polyclonal
The anti-Serotonin antibody IS1007 allows for accturate labeling of 5-HT in cell cultures, tissue sections, and whole mounts prepared with the STAINperfect immunostaining kit A. Our team has validated this anti-5-HT antibody for immunofluorescence applications in crayfish tissues (whole mounts) as well as in mouse primary neurons and brain tissues (both tissue sections and whole mounts).
€449
97 in stock
Pay by online or by purchase order
Pay by credit/debit card or generate a quote at checkout (your products will be sent once you provide us with the purchase order)
Fast Worldwide Shipping with Fedex
We ship your order door-to-door in all countries with FedEx International Priority®.
Worry-free Warranty
Made a mistake ordering ? 30-day free return & refund.
Product damaged during shipping ? Free replacement.
Not getting satisfactory results ? If product did not meet quality standards, customer support offers replacement or refund.
Direct Customer Support
For questions, protocol recommendations, and troubleshooting, you have direct access to the scientist that developed the antibody or ELISA kit you’re using.
Direct customer support is available all business days.
A knowledge base containing protocols, Frequently Asked Questions, and troubleshooting tips is also available online.
They published with this product
You may also like…
Serotonin Antibody for FFPE samples – Rabbit Polyclonal
€449
STAINperfect™ immunostaining kit A
€299€340
L-Tryptophan Antibody – Mouse Monoclonal
€449
Serotonin Antibody – Mouse Monoclonal
€449
IS1007
Serotonin Antibody – Rabbit Polyclonal
Anti-Serotonin pAb – Purified polyclonal antibody for research use only (liquid 50µL)
Specifications
| Product name | Serotonin antibody (5-HT rabbit polyclonal Ab) |
| Synonyms | Anti-5-HT antibody Anti-5-hydroxytryptamine antibody |
| Immunogen | Conjugated 5-HT |
| Specificity | When tested in competitive ELISA, the anti-conjugated Serotonin antibody did not show any significant cross reactivity with 5-Hydroxytryptamine analogs, including Tryptamine and 5-Methoxytryptamine |
| Volume | 50 µL |
| Form | Liquid |
| Purity | Purified anti-serum |
Instructions
| Storage |
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Immunofluorescence (IF) |
Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample (Cell cultures, Whole mounts, Tissue sections) |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
