The clear path to small molecule visualization & quantification

Key features

Format Ready-to-use staining kit
Reactivity Applicable to all species
Validated protocols Cell cultures, tissue sections, whole mounts
Number of stainings Cell cultures : 1x 96 well-plate
Whole mounts : 20 samples
Tissue sections: 40 slides

Citations

Frequently asked questions

SP-A-1000

STAINperfect™ immunostaining kit A

The STAINperfect™ immunostaining kit A provides all necessary reagents and protocols to achieve high-quality stainings with Immusmol’s antibodies. The kit can be combined with over 30 antibodies targeting key neurotransmitters, amino acids, and their downstream metabolites. See compatible antibodies below.

299340

Number of citations :

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A knowledge base containing protocols, Frequently Asked Questions, and troubleshooting tips is also available online. 

They published with this product

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Glycine Antibody – Rabbit Polyclonal

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SP-A-1000

STAINperfect™ immunostaining kit A

STAINperfect immunostaining kit – ICC & IHC staining kit for small molecule imaging

Specifications

Product name STAINperfect™ immunostaining kit A
Description Ready-to-use immunostaining kit providing optimal staining conditions for specific Immusmol’s antibodies
Eligible samples Cell culture, whole mounts, tissues from any species
Number of stainings Cell cultures : 1x 96 well-plate
Whole mounts : 20 samples
Tissue sections: 40 slides
Restriction For research use only

Instructions

Storage
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles
IF in cell cultures
Fixation (15 min), Permeabilization (15 min), Stabilization (15 min), Saturation (30 min), Primary antibody incubation (overnight), Wash and secondary antibody (1h15 min)
IF in whole mounts
Fixation (3.5h), Permeabilization (1h), Stabilization (1h), Saturation (1h), Primary antibody incubation (72h), Secondary antibody (overnight)
IF/IHC in tissue sections

(in vivo studies)

Fixation via intracardiac perfusion & post-fixation (4h), Slicing, Drying slides (40 min), Permeabilization (20 min), Stabilization (70 min), Saturation (30 min), Primary antibody incubation (overnight), Wash and secondary antibody (1h15 min)
Comments Optimal working dilutions must be determined by the end-user
Restrictions For research use only

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