Epinephrine – Norepinephrine ELISA kit – Ultra-Sensitive – Any sample

Ref: BA-E-5400R
CatecholaminesResearch ELISA kits (RUO)ELISA
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Epinephrine-Norepinephrine ELISA package
DatasheetProtocolMSDS

This research Epinephrine/Norepinephrine ELISA kit enables the ultra-sensitive measurement of Adrenaline (Epinephrine) and Noradrenaline (Norepinephrine) in any biological sample. Intended for research use only, the assay features a lower limit of detection at 6.6pg/ml and a minimal sample volume as low as 1µl.

Sample type1 – 750µl, all biological fluids
Samples/kit40 samples in duplicate
SensitivityEpinephrine: 6.6pg/ml;
Norepinephrine: 2.6pg/ml
RangeEpinephrine: 0.5 – 80ng/ml;
Norepinephrine: 0.2 – 32ng/ml
Assay timeSample extraction, acylation and enzymatic conversion (4 h), ELISA overnight
ReactivityReacts with all species
CitationsCited in 3 papers 
Size

2 x 96 wells

924.00€

91 in stock

Quantity
Total

924.00€

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Product overview

Product nameEpinephrine-Norepinephrine ELISA kit – Research
DescriptionEnzyme immunoassay (ELISA) for the ultra-sensitive determination of epinephrine and norepinephrine in biological samples. Research use only
Format2 x 96-well plate
SamplesAny sample
Sample volume1 – 750µl
ReactivityReacts with all species
Standard rangeEpinephrine: 0 / 0.5 – 80ng/ml;
Norepinephrine: 0 / 0.2 – 32ng/ml
Sensitivity

Epinephrine: 6.6pg/ml;
Norepinephrine: 2.6pg/ml

SpecificityNo significant cross-reactivity was observed with Epinephrine and Norepinephrine analogs such as Dopamine, Metanephrine, Normetanephrine, 3-Methoxytyramine, 3-Methoxy-4-hydroxyphenylglycol, Tyramine, Phenylalanine, Caffeinic acid, L-Dopa, Homovanillic acid, Tyrosine and 3-Methoxy-4-hydroxymandelic acid
Assay timeSample preparation 4 hours and ELISA overnight
StorageStore at 2-8°C for to 6 months.
DatasheetsInstructions for use, Material safety datasheet

Sample preparationSample extraction and acylation (1h45), enzymatic conversion (2h)
ELISAEpinephrine/Norepinephrine antiserum overnight incubation, revelation and read steps (1h)
Detailed protocolDownload instructions for use

Product citation

  • Upregulation of the hyperpolarization-activated current increases pacemaker activity of the sinoatrial node and heart rate during pregnancy in mice
    Check the article
    Authors : El Khoury et al., Circulation
    2013-05
  • Acute hypoxia induces hypertriglyceridemia by decreasing plasma triglyceride clearance in mice
    Check the article
    Authors : Jun et al., American journal of Physiology, Endocrinology & Metabolism
    2012-05
  • Pancreatic cancer cells and normal pancreatic duct epithelial cells express an autocrine catecholamine loop that is activated by nicotinic acetylcholine receptors α3, α5 and α7
    Check the article
    Authors : Al-Wadei et al., Molecular Cancer Research
    2011-12