Antibodies & immunoassays for small molecules GABA Monoclonal Antibody
Ref: IS039
Monoclonal AntibodiesICCIFIHCNeuromediators
+Total and GABAergic neuronal networks in mature rat cortical cultures
Image field micrographs of mature (12 DIV-old) rat cortical cultures, illustrating total and GABAergic neuronal networks revealed by MAP2 (in red) and GABA (in green) immunostaining, respectively. GABA immunostaining obtained using whether our monoclonal (#IS039) or polyclonal GABA antibody (#IS1006) with the STAINperfect immunostaining
DatasheetMSDS
Anti-GABA antibody (#IS039) is a mouse monoclonal antibody specifically selected by competitive ELISA for its affinity and specificity features. Used with the STAINperfect immunostaining kit A through appropriate protocols, our antibody directly labels GABA and allows to evidence, in the same way as with our validated reference rabbit polyclonal anti-GABA antibody (#IS1006), GABAergic systems – cell bodies and neuritic network, in whole mounts, cell culture and tissue sections.
| Clonality | Monoclonal antibody (clone 7F52B41B4) |
| Host | Mouse |
| Reactivity | Reacts with all species |
| Applications | IHC / IF |
| Tested samples | Whole mounts, cell culture, tissue sections |
| Staining procedure | STAINperfect immunostaining kit A |
| Format | 50µL |
| Product name | GABA antibody – mouse mAb |
| Synonyms | Anti-g-Aminobutyric acid antibody
Anti-gamma-Aminobutyric acid antibody |
| Immunogen | Conjugated GABA |
| Specificity | When tested in competitive ELISA, the anti-conjugated GABA antibody did not show any significant cross reactivity with gamma-Aminobutyric acid analogs, including beta-Alanine and L.Glutamate |
| Volume | 50µL |
Storage
| Form | Liquid |
| Purity | Purified IgG1 (lambda) |
| Storage |
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Material safety datasheet | Download MSDS |
IF – Cell cultures, Whole mounts, Tissue sections
| Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample | |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
| Full protocol | Download STAINperfect protocol for GABA staining |
| Protocols-at-a-glance |
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| Complete Instructions for Use |
Protocol-at-a-glance for cell cultures |
Protocol-at-a-glance for whole mounts |
Protocol-at-a-glance for tissue sections |
Enregistrer
Antibody not yet cited. Submit an article and get a 10% discount.
Selected articles on GABA:
- Liu X, Wang Q, Haydar TF, Bordey A. Nonsynaptic GABA signaling in postnatal subventricular zone controls proliferation of GFAP-expressing progenitors. Nat Neurosci. 2005 Sep;8(9):1179-87. Epub 2005 Aug 14.
- Lawrence JJ. Cholinergic control of GABA release: emerging parallels between neocortex and hippocampus. Trends Neurosci. 2008 Jul;31(7):317-27. doi: 10.1016/j.tins.2008.03.008. Epub 2008 Jun 13.
- Baulac S, Huberfeld G, Gourfinkel-An I, Mitropoulou G, Beranger A, Prud’homme JF, Baulac M, Brice A, Bruzzone R, LeGuern E. First genetic evidence of GABA(A) receptor dysfunction in epilepsy: a mutation in the gamma2-subunit gene. Nat Genet. 2001 May;28(1):46-8.
