Antibodies & immunoassays for small molecules Kynurenic acid Antibody – Mouse Monoclonal
Ref: IS010
Kynurenine pathwayMonoclonal AntibodiesICCIFIHC
+Kynurenic acid detection in human brain by IF (mouse mAb)
Immufluorescence shows kynurenic acid accumulation in the cytoplasm of glial cells in human caudate-putamen. Paraffin-embedded tissue section was subjected to pH=6 antigen retrieval followed by overnight incubation with primary anti-Kynurenic acid antibody (dilution 1/250). After incubation with fluorescent dye-conjugated secondary Ab, epifluorescence microscopy (100X) was used to visualize the staining.
DatasheetMSDS
The monoclonal IS010 anti-Kynurenic acid antibody was validated for IHC and IF in human caudate putamen tissues. Competitive ELISA demonstrated the antibody to be highly affine and specific.
| Clonality | Monoclonal antibody (clone 4G12-A12) |
| Host | Mouse |
| Validated applications | IHC / IF |
| Specie reactivity | Reacts with all species |
| References | Not yet cited to our knowledge. Submit content and get a 10% discount! |
| Format | 50µL |
| References | Cited in literature |
Product overview
| Product name | Kynurenic acid antibody |
| Synonyms | Kinurenic acid antibody
4-Hydroxyquinoline-2-carboxylic acid antibody KYNA antibody |
| Immunogen | Conjugated kynurenic acid |
| Isotype | IgG1 k chain |
| Clone | clone 4G12-A12 |
| Specificity | When tested in competitive ELISA, the anti-Kynurenic antibody 4G12-A12 did not show any significant cross reactivity with Quinaldic, Xanthurenic, Anthranilic, Picolinic or Quinolinic acid conjugates |
Storage
| Form | Liquid |
| Purity | Purified IgG |
| Concentration | 0,5mg/ml |
| Storage | Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Material safety datasheet | Download MSDS |
| Immunohistochemistry (IHC) | Dilute at 1:200-1:2000. Perform heat antigen retrieval (pH=6) before initiating IHC staining protocol on paraffin-embedded and frozen sections |
| Immunofluorescence (IF) | 1:100-1:1000 on paraffin-embedded and frozen sections. Before staining, perform heat antigen retrieval |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
Literature citations
- Atheroma plaque microenvironment stimulates kynurenine production by macrophages to induce endothelial adhesion molecules in the context of atherogenesis
Check the article
Authors : Dongen et al., bioRxiv
2023-07
