Antibodies & immunoassays for small molecules Metanephrine-Normetanephrine ELISA kit
Urine samples
Ref: BA-E-8600
CatecholaminesDiagnostic ELISA kits (IVD)ELISA
+Urine Metanephrines ELISA
DatasheetProtocolMSDS
This Metanephrines ELISA kit enables to quantify metanephrine and normetanephrine in urine samples within 2 hours. Intended for in vitro diagnostics, this quantitative immunoassay demonstrated good correlation with HPLC.
| Sample type | 25µl urine |
| Samples/kit | 40 samples in duplicate |
| Sensitivity | Metanephrine: 13 ng/ml Normetanephrine: 23 ng/ml |
| Range | Metanephrine: 20 – 2 000 ng/ml Normetanephrine: 30 – 3 000ng/ml |
| Assay time | Sample hydrolysis and acetylation (45 min), ELISA (1h) |
| Reactivity | Reacts with all species |
| Citations | 2 |
Product overview
| Product name | Metanephrine-Normetanephrine ELISA kit – Urine- Fast-Track |
| Description | In vitro diagnostics enzyme immunoassay (ELISA) for the fast determination of metanephrines (metadrenaline and normetadrenaline) in urine samples |
| Labels | IVD, CE |
| Format | 96-well plate |
| Samples | Urine |
| Sample volume | 25µL |
| Reactivity | Reacts with all species |
| Standard range | 0 / 20 – 2 000ng/ml – Metanephrine 0 / 30 – 3 000ng/ml – Normetanephrine |
| Sensitivity |
13ng/ml – Metanephrine |
| Specificity | No significant cross-reactivity was observed with Metanephrines analogs such as 3-methoxytyramine, Adrenaline, Noradrenaline, Dopamine, Vanillic mandelic acid, L-Dopa, Homovanillic acid, L-Tyrosine and Tyramine |
| Assay time | Sample hydrolysis and acetylation 45 min and ELISA |
| Storage | Store at 2-8°C for to 6 months |
| Datasheets | Instructions for use, Material safety datasheet |
| Kits intended for in-vitro diagnostic (IVD) are not available for the US-market.
In contrast to kits for in-vitro-diagnostic (IVD), kits meant for research use only (RUO) do have a R in the last position of the catalog number. |
| Sample collection & storage | Spontaneous or 24-hours urine, collected in a bottle containing 10-15 mL of 6 M HCl. Storage at -20°C. for long periods (up to 6 months) |
| Sample preparation | Sample hydrolysis (30 min) and acetylation (15min) |
| ELISA | Metanephrine antiserum incubation (30 min), revelation and read steps (30h) |
| Detailed protocol | Download instructions for use |
Product citation
- Quantification of normetanephrine in canine urine using ELISA: evaluation of factors affecting results
Check the article
Authors : Hoglund et al., Journal of veterinary diagnosic investigation
2021-10 - Plasma and urinary metanephrines determined by an enzyme immunoassay, but not serum chromogranin A for the diagnosis of pheochromocytoma in patients with adrenal mass
Check the article
Authors : Unger et al., Experimental & clinical endocrinology & diabetes
2012-06 - Blood pressure changes after automatic and fixed CPAP in obstructive sleep apnea: relationship with nocturnal sympathetic activity
Check the article
Authors : Marrone et al., Clinical & experimental hypertension
2011-05
