Metanephrine-Normetanephrine ELISA kit – Fast – Urine samples

Ref: BA-E-8600
CatecholaminesDiagnostic ELISA kits (IVD)Research ELISA kits (RUO)ELISA
DatasheetProtocolMSDS

This Metanephrines ELISA kit enables to quantify metanephrine and normetanephrine in urine samples within 2 hours. Intended for in vitro diagnostics & Research, this quantitative immunoassay demonstrated good correlation with HPLC.

Sample type25µl urine
Samples/kit40 samples in duplicate
SensitivityMetanephrine: 13 ng/ml
Normetanephrine: 23 ng/ml
RangeMetanephrine: 20 – 2 000 ng/ml
Normetanephrine: 30 – 3 000ng/ml
Assay timeSample hydrolysis and acetylation (45 min), ELISA (1h)
ReactivityReacts with all species
CitationsCited in 3 papers 
Size

2 x 96 wells

Regulatory status

In Vitro Diagnostics (EU), Research Use Only

836.00

Clear
Quantity
Total

836.00

Worldwide shipping

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Product overview

Product nameMetanephrine-Normetanephrine ELISA kit – Urine- Fast-Track
DescriptionIn vitro diagnostics enzyme immunoassay (ELISA) for the fast determination of metanephrines (metadrenaline and normetadrenaline) in urine samples
LabelsIVD, CE
Format96-well plate
SamplesUrine
Sample volume25µL
ReactivityReacts with all species
Standard range0 / 20 – 2 000ng/ml – Metanephrine
0 / 30 – 3 000ng/ml – Normetanephrine
Sensitivity

13ng/ml – Metanephrine
23ng/ml – Normetanephrine

SpecificityNo significant cross-reactivity was observed with Metanephrines analogs such as 3-methoxytyramine, Adrenaline, Noradrenaline, Dopamine, Vanillic mandelic acid, L-Dopa, Homovanillic acid, L-Tyrosine and Tyramine
Assay timeSample hydrolysis and acetylation 45 min and ELISA
StorageStore at 2-8°C for to 6 months
DatasheetsInstructions for use, Material safety datasheet

 

Sample collection & storageSpontaneous or 24-hours urine, collected in a bottle containing 10-15 mL of 6 M HCl. Storage at -20°C. for long periods (up to 6 months)
Sample preparationSample hydrolysis (30 min) and acetylation (15min)
ELISAMetanephrine antiserum incubation (30 min), revelation and read steps (30h)
Detailed protocolDownload instructions for use

Product citation

  • Quantification of normetanephrine in canine urine using ELISA: evaluation of factors affecting results
    Check the article
    Authors : Hoglund et al., Journal of veterinary diagnosic investigation
    2021-10
  • Plasma and urinary metanephrines determined by an enzyme immunoassay, but not serum chromogranin A for the diagnosis of pheochromocytoma in patients with adrenal mass
    Check the article
    Authors : Unger et al., Experimental & clinical endocrinology & diabetes
    2012-06
  • Blood pressure changes after automatic and fixed CPAP in obstructive sleep apnea: relationship with nocturnal sympathetic activity
    Check the article
    Authors : Marrone et al., Clinical & experimental hypertension
    2011-05

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